Komiya Akiko, Matsui Toshihiro, Horie Koichiro, Fukuda Hidefumi, Nogi Shinichi, Iwata Kanako, Tsuno Hirotaka, Ogihara Hideki, Futami Hidekazu, Ikenaka Tatsuoh, Kawakami Misato, Furukawa Hiroshi, Hashimoto Atsushi, Tohma Shigeto
Department of Clinical Laboratory, Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan.
Mod Rheumatol. 2014 Sep;24(5):770-4. doi: 10.3109/14397595.2013.871108. Epub 2014 Jan 28.
To evaluate the utility of neutrophil CD64 as a marker for monitoring the activity of nontuberculous mycobacteria (NTM) infection in patients with rheumatoid arthritis (RA).
We compared neutrophil CD64 expression in nine RA patients with NTM infection in the active and inactive phase of NTM disease chronologically. "Active phase" was here defined as present in patients admitted to hospital to receive intensive treatment for NTM, as well as outpatients with an infectious episode showing positive acid- and alcohol-fast bacillus (AFB) staining of sputa (Grade 2-3) who needed to start treatment for NTM with a multiple antibiotics regimen. The cut-off value for CD64 positivity was 2000 molecules/cell.
Neutrophils from patients with active-phase NTM infection expressed high levels of CD64 with a mean ± SEM of 7335 ± 784 molecules/cell. However, during the inactive phase of disease, this was significantly lower (1481 ± 103 molecules/cell, p < 0.001). The sensitivity and specificity of neutrophil CD64 to detect active-phase NTM infection was 96.3% and 84.6%, respectively. Expression of neutrophil CD64 was not affected by disease activity of the RA itself.
Neutrophil CD64 is useful for monitoring disease activity in NTM infection of patients with RA.
评估中性粒细胞CD64作为监测类风湿关节炎（RA）患者非结核分枝杆菌（NTM）感染活动度标志物的效用。
我们按时间顺序比较了9例患有NTM感染的RA患者在NTM疾病活动期和非活动期的中性粒细胞CD64表达情况。“活动期”在此定义为因NTM而住院接受强化治疗的患者，以及痰液抗酸杆菌（AFB）染色呈阳性（2 - 3级）且需要开始使用多种抗生素方案治疗NTM的感染发作期门诊患者。CD64阳性的临界值为2000分子/细胞。
活动期NTM感染患者的中性粒细胞表达高水平的CD64，平均±标准误为7335±784分子/细胞。然而，在疾病非活动期，这一水平显著降低（1481±103分子/细胞，p < 0.001）。中性粒细胞CD64检测活动期NTM感染的敏感性和特异性分别为96.3%和84.6%。中性粒细胞CD64的表达不受RA自身疾病活动度的影响。
中性粒细胞CD64可用于监测RA患者NTM感染的疾病活动度。
