Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602.
ACS Appl Mater Interfaces. 2014 Feb 26;6(4):2479-85. doi: 10.1021/am404907c. Epub 2014 Feb 5.
Functionalization of amine derivatized glass slides with a poly(maleic anhydride)-based comb-copolymer to facilitate stretching, aligning, and imaging of individual dsDNA chains is presented. The polymer-coated surface is hydrophobic due to the presence of the long alkyl side chains along the polymer backbone. The surface is also characterized by low roughness and a globular morphology. Stretched and aligned bacteriophage λ-DNA chains were obtained using a robust method based on stretching by a receding water meniscus at pH 7.8 without the need for small droplet volumes or precoating the surface with additional layers of (bio)molecules. Although the dye to DNA base pairs ratio did not influence substantially the stretching length distributions, a clear peak at stretching lengths close to the contour length of the dsDNA is visible at larger staining ratios.
介绍了一种聚（马来酸酐）基梳状共聚物对胺衍生玻璃载玻片的功能化，以方便拉伸、对齐和成像单个 dsDNA 链。由于聚合物主链上存在长烷基侧链，因此聚合物涂层表面具有疏水性。该表面还具有低粗糙度和球形形貌的特点。使用基于在 pH 值为 7.8 时通过退去的水弯月面拉伸的稳健方法获得了拉伸和对齐的噬菌体 λ-DNA 链，而不需要小液滴体积或用额外的（生物）分子层预先涂覆表面。尽管染料与 DNA 碱基对的比例并没有显著影响拉伸长度分布，但在较大的染色比下，在接近 dsDNA 轮廓长度的拉伸长度处可以看到一个清晰的峰。
