Sorenson S C, Asch B B, Connolly J L, Burstein N A, Asch H L
Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, NY 14263.
J Pathol. 1987 Oct;153(2):151-62. doi: 10.1002/path.1711530208.
Two monoclonal antikeratin antibodies, AE1 and AE3, were used in indirect immunocytochemistry to examine keratin expression in normal, benign proliferative, and malignant human breast epithelium. Both antibodies reacted strongly with most luminal cells in ducts and acini of normal gland. While AE1 did not stain myoepithelium, AE3 recognized myoepithelial cells of ducts but not acini, implying a cytoskeletal difference between the myoepithelium of these two components. Moreover, the antibodies reacted differently with the myoepithelium of intracanalicular as compared with pericanalicular types of fibroadenomas. Tumour cells of infiltrating ductal carcinomas with a prominent intraductal component stained more homogeneously with AE1 and AE3 than those without intraductal growth. The results provide evidence for two phenotypes of myoepithelial cells and for the presence of cryptic keratin epitopes in human breast epithelial cells. The finding that neither AE1 nor AE3 is a universal detector of these cells has important clinical and experimental implications.
两种单克隆抗角蛋白抗体AE1和AE3被用于间接免疫细胞化学，以检测人正常乳腺上皮、良性增生性乳腺上皮及恶性乳腺上皮中的角蛋白表达。两种抗体均与正常腺体导管和腺泡中的大多数管腔细胞发生强烈反应。AE1不染色肌上皮细胞，而AE3可识别导管的肌上皮细胞，但不能识别腺泡的肌上皮细胞，这意味着这两种结构的肌上皮细胞在细胞骨架方面存在差异。此外，与管周型纤维腺瘤相比，这两种抗体与管内型纤维腺瘤肌上皮细胞的反应不同。具有显著导管内成分的浸润性导管癌的肿瘤细胞，与无导管内生长的肿瘤细胞相比，用AE1和AE3染色更均匀。这些结果为肌上皮细胞的两种表型以及人乳腺上皮细胞中隐匿性角蛋白表位的存在提供了证据。AE1和AE3都不是这些细胞的通用检测剂这一发现具有重要的临床和实验意义。
