2007-2013 年澳大利亚维多利亚州患者脑脊液中肠道病毒的检测和基因分型。

Detection and genotyping of enteroviruses in cerebrospinal fluid in patients in Victoria, Australia, 2007-2013.

机构信息

Virology Department, Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia.

出版信息

J Med Virol. 2014 Sep;86(9):1609-13. doi: 10.1002/jmv.23885. Epub 2014 Jan 29.

Abstract

Genotyping by VP1 fragment polymerase chain reaction (PCR) and nucleic acid sequencing to detect enterovirus (EV) genotypes was performed directly on 729 EV PCR positive cerebrospinal fluid (CSF) samples collected between 2007 and 2012 from Victorian hospital inpatients. The overall genotype identification rate from CSF-positive material was 43%. The four most common genotypes identified were Echovirus 6 (24%), Echovirus 30 (17%), Echovirus 25 (10%), and Coxsackievirus A9 (10%), together comprising 61% of all EVs typed. The seasonal distribution of all EVs identified followed the recognized pattern of mainly summer epidemics. Three of the four predominant genotypes were present in each of the 6 years in which the study was conducted, with 20 other EV genotypes also detected, often in only a single year. Genotyping of EVs directly in CSF is faster, simpler and more sensitive than traditional virus neutralization assays performed on EV positive samples.

摘要

对 2007 年至 2012 年间收集的 729 份来自维多利亚州住院患者的肠病毒（EV）PCR 阳性脑脊液（CSF）样本，直接通过 VP1 片段聚合酶链反应（PCR）和核酸测序进行基因分型，以检测 EV 基因型。从 CSF 阳性标本中进行的总体基因型鉴定率为 43%。鉴定出的四种最常见的基因型是肠道病毒 6 型（24%）、肠道病毒 30 型（17%）、肠道病毒 25 型（10%）和柯萨奇病毒 A9 型（10%），这四种病毒占所有鉴定出的 EV 的 61%。所有鉴定出的 EV 的季节性分布符合主要夏季流行的公认模式。在开展研究的 6 年中，这四种主要基因型中的每一种都存在，还检测到其他 20 种 EV 基因型，这些基因型往往只在一年中出现。与在 EV 阳性样本上进行的传统病毒中和试验相比，直接在 CSF 中对 EV 进行基因分型更快、更简单、更灵敏。