Zhang Yuan, Liu Yukun, Sun Yan, Liu Qingmei, Wang Xiaoyan, Li Zhi, Hao Jie
College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, Shaanxi, China.
Curr Microbiol. 2014 Jun;68(6):685-92. doi: 10.1007/s00284-014-0529-4. Epub 2014 Jan 29.
Antimicrobial peptides are the promising candidates for withstanding multidrug-resistant bacteria (MDRB) which were caused by the misuse and extensive use of antibiotics. In this research, in vitro activities of one antimicrobial cationic peptide, brevinin-2CE alone and in combination with five kinds of antibiotics were assessed against clinical isolates of extended-spectrum β-lactamase-producing Escherichia coli and methicillin-resistant Staphylococcus aureus. The results showed that most of the combination groups had synergistic effects. Also, it was obvious that brevinin-2CE had more rapid and severe action on the tested MDRBs which demonstrated that brevinin-2CE and the antibiotics had different antimicrobial mechanisms. Thus, it was presumed that the antimicrobial peptides destroyed the bacterial cells via pore formation mechanisms which lead to the increasing of membrane permeability; and then the other compounds like antibiotics might enter into the cells and accomplish the antimicrobial activities more rapidly and efficiently.
抗菌肽是应对因抗生素滥用和广泛使用导致的多重耐药菌（MDRB）的有潜力的候选物。在本研究中，评估了一种抗菌阳离子肽brevinin-2CE单独以及与五种抗生素联合使用时，对产超广谱β-内酰胺酶的大肠埃希菌和耐甲氧西林金黄色葡萄球菌临床分离株的体外活性。结果表明，大多数联合组具有协同作用。此外，很明显brevinin-2CE对测试的多重耐药菌作用更快且更强烈，这表明brevinin-2CE和抗生素具有不同的抗菌机制。因此，推测抗菌肽通过形成孔道机制破坏细菌细胞，导致膜通透性增加；然后像抗生素这样的其他化合物可能进入细胞并更快速有效地完成抗菌活性。
