Yan Ling-geng, Ruan Jun-shan, Zhang Lei, Fan Fang-tian, Zhang Feng, Wang Ai-yun, Zheng Shi-zhong, Zeng Li, Li Wen-lin, Lu Yin
Department of Clinical Pharmacy, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210029, China.
Chin J Integr Med. 2015 Apr;21(4):286-90. doi: 10.1007/s11655-013-1540-5. Epub 2014 Jan 28.
To study the effect of aqueous extract of several kinds of herbs on human platelet aggregation and expression of P-selectin in vitro.
Blood was collected from volunteers. Effects of the prepared water extracts of herbs on platelet aggregation were monitored on a Packs-4 aggregometer. The fluorescence intensity of water extracts of Caulis Spatholobi, Flos Carthami and Rhizoma Curcumae on the expression of P-selectin in human platelets of healthy persons was measured with flow cytometry.
Out of several herbs investigated, Flos Carthami and Rhizoma Curcumae potently inhibited platelet aggregation after incubation with platelet-rich plasma (PRP) for 15 min. Caulis Spatholobi Flos Carthami and Rhizoma Curcumae inhibited adenosine-5'-diphosphate (ADP) or platelet activating factor (PAF)-induced platelet aggregation in PRP in a dose-dependent manner. In contrast to Flos Carthami and Rhizoma Curcumae, Caulis Spatholobi could not inhibit thrombin-induced platelet aggregation. Despite its inability to inhibit thrombin-induced platelet aggregation in PRP, Caulis Spatholobi had a greater anti-aggregating activity in PRP induced by ADP or PAF. Caulis Spatholobi and Flos Carthami showed significant inhibitory effects on the expression of P-selectin.
Caulis Spatholobi, Flos Carthami and Rhizoma Curcumae have potent anti-platelet properties, and their inhibitory actions are mediated via different mechanisms. Caulis Spatholobi inhibited ADP-induced platelet aggregation but not by thrombin, indicating that its mechanism of action might be independent of the thromboxane pathway. The effect of Caulis Spatholobi and Flos Carthami were associated with suppressing the expression of P-selectin.
研究几种草药水提取物对人血小板聚集及P-选择素表达的体外影响。
采集志愿者血液。用Packs-4血小板聚集仪监测所制备的草药水提取物对血小板聚集的影响。采用流式细胞术测定鸡血藤、红花和莪术水提取物对健康人血小板中P-选择素表达的荧光强度。
在所研究的几种草药中，红花和莪术与富血小板血浆（PRP）孵育15分钟后能有效抑制血小板聚集。鸡血藤、红花和莪术以剂量依赖方式抑制PRP中腺苷-5'-二磷酸（ADP）或血小板活化因子（PAF）诱导的血小板聚集。与红花和莪术不同，鸡血藤不能抑制凝血酶诱导的血小板聚集。尽管鸡血藤不能抑制PRP中凝血酶诱导的血小板聚集，但它对ADP或PAF诱导的PRP具有更强的抗聚集活性。鸡血藤和红花对P-选择素的表达有显著抑制作用。
鸡血藤、红花和莪术具有较强的抗血小板特性，它们的抑制作用通过不同机制介导。鸡血藤抑制ADP诱导的血小板聚集，但不抑制凝血酶诱导的聚集，表明其作用机制可能独立于血栓素途径。鸡血藤和红花的作用与抑制P-选择素的表达有关。
