EspA-紧密黏附素嵌合蛋白，一种抗大肠杆菌O157:H7的候选疫苗。
EspA-Intimin chimeric protein, a candidate vaccine against Escherichia coli O157:H7.
作者信息
Rad Hamid Sedighian, Mousavi Seyed Latif, Rasooli Iraj, Amani Jafar, Nadooshan Moohamad Reza Jalali
机构信息
Applied Microbiology Research Center, Baqiyatallah Medical Science University, Tehran.
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.
出版信息
Iran J Microbiol. 2013 Sep;5(3):244-51.
BACKGROUND AND OBJECTIVE
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important enteric pathogen in human causing bloody or nonbloody diarrhea, which may be complicated by hemolytic uremic syndrome (HUS). Cattle are an important reservoir of EHEC. This research aims at vaccination with a divalent chimer protein composed of EspA120 and Intimin 282 and its preventive effect of EHEC O157 colonization in mice rectal epithelium.
MATERIALS AND METHODS
A divalent recombinant EspA-Intimin (EI) protein containing EspA120 and Intimin280 attached with a linker was amplified from a trivalent construct and cloned in pET-28a (+) vector. The immunization was conducted in mice after expression and purification of the recombinant EI (rEI).
RESULTS
Mice subcutaneously immunized with rEI, elicited significant rEI specific serum IgG antibodies and showed significantly decreased E.coli O157:H7 shedding compared to the control group.
CONCLUSION
The chimeric recombinant protein induced strong humoral response as well as protection against oral challenges with live E.coli O157:H7.
背景与目的
肠出血性大肠杆菌（EHEC）O157:H7是引起人类血性或非血性腹泻的重要肠道病原体，可能并发溶血尿毒综合征（HUS）。牛是EHEC的重要储存宿主。本研究旨在用由EspA120和紧密黏附素282组成的二价嵌合蛋白进行疫苗接种及其对小鼠直肠上皮中EHEC O157定植的预防作用。
材料与方法
从三价构建体中扩增出含EspA120和紧密黏附素280并连接有接头的二价重组EspA-紧密黏附素（EI）蛋白，并克隆到pET-28a（+）载体中。重组EI（rEI）表达纯化后在小鼠中进行免疫接种。
结果
与对照组相比，用rEI皮下免疫的小鼠产生了显著的rEI特异性血清IgG抗体，且大肠杆菌O157:H7的粪便排出量显著降低。
结论
嵌合重组蛋白诱导了强烈的体液反应以及对活的大肠杆菌O157:H7口服攻击的保护作用。
Zotero 插件