Jakobsen P H, Jepsen S, Agger R
Malaria Research Laboratory, Treponematoses Department, Statens Seruminstitut, Copenhagen, Denmark.
Parasitol Res. 1987;73(6):518-23. doi: 10.1007/BF00535326.
Several murine monoclonal antibodies were raised against purified soluble Plasmodium falciparum antigens from the asexual blood stage. The monoclonal antibodies were purified from ascites by preparative agarose gel electrophoresis and tested for inhibitory activities against P. falciparum in vitro cultures. One monoclonal antibody, HATR 2-4, showed an isolate-specific growth inhibition of P. falciparum in vitro cultures. The antibody reacted in immunoblotting with bands of 250 and 57 kilo dalton (kdalton). Another monoclonal antibody, HATR 2-8, showed growth inhibition of several geographically distinct P. falciparum isolates. HATR 2-8 reacted in immunoblotting with bands of 250 and 74 kdalton. Heating of the antigens destroyed the reactivity of HATR 2-8. The monoclonal antibodies HATR 2-4 and HATR 2-8 probably recognize different epitopes on the same antigen. This antigen circulates in the plasma of some patients with P. falciparum parasitaemia.
针对来自恶性疟原虫无性血液阶段的纯化可溶性抗原，制备了几种鼠单克隆抗体。通过制备性琼脂糖凝胶电泳从腹水中纯化单克隆抗体，并在体外培养中测试其对恶性疟原虫的抑制活性。一种单克隆抗体HATR 2-4在体外培养中对恶性疟原虫表现出分离株特异性生长抑制作用。该抗体在免疫印迹中与250和57千道尔顿（kdalton）的条带发生反应。另一种单克隆抗体HATR 2-8对几种地理上不同的恶性疟原虫分离株表现出生长抑制作用。HATR 2-8在免疫印迹中与250和74 kdalton的条带发生反应。抗原加热会破坏HATR 2-8的反应性。单克隆抗体HATR 2-4和HATR 2-8可能识别同一抗原上的不同表位。这种抗原在一些恶性疟原虫血症患者的血浆中循环。
