Pei Zhenhua, Wang Baokui, Zhang Fengqiu, Niu Zhongying, Shi Shenggen, Cannon Richard D, Mei Li
Department of Stomatology, 306th Hospital of People's Liberation Army, Beijing, China.
J Periodontol. 2014 Sep;85(9):1283-90. doi: 10.1902/jop.2014.130635. Epub 2014 Jan 30.
Periodontitis is the most common cause of tooth loss in adults. Periodontal ligament cell (PLC)-based therapy is considered one of the most promising methods in periodontal tissue regeneration. The traditional Chinese medicine baicalin has been shown to possess antimicrobial and anti-inflammatory activities and enhance cell proliferation and alkaline phosphatase activity. The aim of this study is to investigate the response of human PLCs (HPLCs) to baicalin.
The effect of baicalin on cultured HPLC proliferation was measured with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The effect of baicalin on the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-κB ligand (RANKL), core binding factor α1 (Cbfα1), and osteocalcin (OC) was determined by quantitative real-time polymerase chain reaction and immunodetection.
Baicalin at a concentration of 0.01 μg/mL promoted HPLC proliferation, upregulated OPG messenger RNA (mRNA) and protein expression, and downregulated RANKL mRNA and protein expression. In addition to reducing the RANKL/OPG expression ratio significantly, it also increased Cbfα1 and OC mRNA and protein expression.
Baicalin showed multifaceted regulation of genes with important roles in tissue growth and differentiation, and thus it has the potential to be a promising candidate for HPLC-based periodontal regeneration therapy.
牙周炎是成年人牙齿缺失的最常见原因。基于牙周膜细胞（PLC）的治疗被认为是牙周组织再生中最有前景的方法之一。中药黄芩苷已被证明具有抗菌和抗炎活性，并能增强细胞增殖和碱性磷酸酶活性。本研究的目的是探讨人牙周膜细胞（HPLC）对黄芩苷的反应。
用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法检测黄芩苷对培养的HPLC增殖的影响。通过定量实时聚合酶链反应和免疫检测法测定黄芩苷对骨保护素（OPG）、核因子κB受体活化因子配体（RANKL）、核心结合因子α1（Cbfα1）和骨钙素（OC）表达的影响。
浓度为0.01μg/mL的黄芩苷促进HPLC增殖，上调OPG信使核糖核酸（mRNA）和蛋白表达，下调RANKL mRNA和蛋白表达。除显著降低RANKL/OPG表达比值外，还增加Cbfα1和OC mRNA及蛋白表达。
黄芩苷对在组织生长和分化中起重要作用的基因具有多方面调节作用，因此它有可能成为基于HPLC的牙周再生治疗的有前景的候选药物。
