State Key Laboratory of Oral Diseases Department of Conservative Dentistry, West China College of Stomatology, Sichuan University, Chengdu, China.
Int Endod J. 2012 Jan;45(1):68-75. doi: 10.1111/j.1365-2591.2011.01949.x. Epub 2011 Sep 8.
To investigate the expression levels of the receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) in human periodontal ligament fibroblasts (PDLs) when stimulated with heat.
Periodontal ligament fibroblasts were subjected to various temperature increases for 5 min and then maintained at 37 °C. After that, cell viability was determined using an MTT assay. The expression levels of RANKL and OPG were investigated using real-time RT-PCR and ELISA. As a control, the cells were cultured at 37 °C. Data were analysed using one-way anova at a significant level of P = 0.05. Results Cell viability was reduced significantly in the heated groups (P < 0.05). Heat stress downregulated the mRNA expression levels of RANKL and OPG (P < 0.05). When the cells were heated at 39 °C, the protein release of OPG was increased (P < 0.05). There were no significant differences between the heated groups and the control in the release of soluble RANKL (P > 0.05). The relative RANKL/OPG expression ratios were decreased at 39, 43 and 50 °C (P < 0.05), but increased at 47 °C (P < 0.05).
Heat influenced the balance between RANKL and OPG in PDLs. Low heat downregulated their relative ratio, whilst high heat upregulated it.
研究热刺激对人牙周膜成纤维细胞（PDLs）中核因子-κB 受体激活物配体（RANKL）和护骨素（OPG）表达水平的影响。
将牙周膜成纤维细胞分别在不同温度下加热 5 min，然后维持在 37°C。之后，通过 MTT 检测细胞活力。采用实时 RT-PCR 和 ELISA 检测 RANKL 和 OPG 的表达水平。作为对照，细胞在 37°C 下培养。采用单因素方差分析，P 值为 0.05。结果：加热组细胞活力显著降低（P < 0.05）。热应激下调 RANKL 和 OPG 的 mRNA 表达水平（P < 0.05）。当细胞加热至 39°C 时，OPG 的蛋白释放增加（P < 0.05）。在加热组和对照组之间，可溶性 RANKL 的释放没有显著差异（P > 0.05）。在 39°C、43°C 和 50°C 时，RANKL/OPG 的相对表达比值降低（P < 0.05），但在 47°C 时升高（P < 0.05）。
热刺激影响了 PDLs 中 RANKL 和 OPG 之间的平衡。低热下调其相对比值，而高热则上调其相对比值。
