Fagher B, Sjögren A, Monti M
Department of Internal Medicine, University Hospital, Lund, Sweden.
Acta Physiol Scand. 1987 Nov;131(3):355-60. doi: 10.1111/j.1748-1716.1987.tb08250.x.
Thermogenesis in human skeletal muscle was monitored by measurement of heat production using perfusion microcalorimetry. Heat production significantly correlated with relative body weight. The energy expenditure of the Na-K-pump (delta P), assessed after inhibition by ouabain in Krebs-Ringer phosphate buffer containing glucose and insulin, amounted to 6% of the total heat production in vastus lateralis muscle. Muscle potassium positively correlated with delta P (r = 0.84, P less than 0.005). For rectus abdominis muscle delta P was 8-15%; 95% confidence interval for the difference was 3-5% when comparison was made with vastus lateralis. The finding of a positive relationship between delta P and muscle magnesium (r = 0.68, P less than 0.04) is possibly explained by the dependence of ATP hydrolysis on internal magnesium. Our data on resting thermogenesis in small muscle samples agree to previous estimates of O2 consumption in human skeletal muscle in vivo.
通过使用灌注微量热法测量产热来监测人体骨骼肌的产热情况。产热与相对体重显著相关。在含有葡萄糖和胰岛素的 Krebs-Ringer 磷酸盐缓冲液中用哇巴因抑制后评估的钠钾泵（δP）的能量消耗占股外侧肌总产热的 6%。肌肉钾与 δP 呈正相关（r = 0.84，P < 0.005）。腹直肌的 δP 为 8 - 15%；与股外侧肌相比时，差异的 95%置信区间为 3 - 5%。δP 与肌肉镁之间呈正相关（r = 0.68，P < 0.04）这一发现可能是由于 ATP 水解对细胞内镁的依赖性所致。我们关于小肌肉样本静息产热的数据与之前对人体骨骼肌体内氧气消耗的估计一致。
