Parisod Christian, Salmon Armel, Ainouche Malika, Grandbastien Marie-Angèle
Laboratory of Evolutionary Botany, Biology Institute, University of Neuchâtel, Neuchâtel, Switzerland.
Methods Mol Biol. 2014;1112:211-7. doi: 10.1007/978-1-62703-773-0_14.
Transposable elements (TE) represent a major fraction of eukaryotic genomes and play many roles in plant epigenetics. In this chapter, we describe the use of Sequence-Specific Amplified Polymorphism (SSAP) as a reliable Transposon Display technique applicable for use in many plant species. We also discuss the interpretation of SSAP data and associated risks. This technique has potential to allow rapid screening of plant populations, especially in nonmodel or wild species.
