Parisod Christian
Laboratory of Evolutionary Botany, Biology Institute, University of Neuchâtel, Rue Emile-Argand 11, 2009, Neuchâtel, Switzerland.
Methods Mol Biol. 2017;1456:243-250. doi: 10.1007/978-1-4899-7708-3_19.
Taking transposable elements into consideration in surveys of genetic and epigenetic variation remains challenging in species lacking a high-quality reference genome. Here, molecular techniques reducing genome complexity and specifically targeting restructuring and methylation changes in TE genome fractions are described. In particular, methyl-sensitive transposon display (MSTD) uses isoschizomers and PCR amplifications to assess the methylation environment of TE insertions. MSTD offers reliable insights into genome-wide epigenetic changes associated with TEs, especially when used together with similar techniques tracking random sequences.
在缺乏高质量参考基因组的物种中，在遗传和表观遗传变异调查中考虑转座元件仍然具有挑战性。本文描述了降低基因组复杂性并专门针对转座元件基因组部分的重组和甲基化变化的分子技术。特别是，甲基敏感转座子展示（MSTD）使用同裂酶和PCR扩增来评估转座子插入的甲基化环境。MSTD能够可靠地洞察与转座元件相关的全基因组表观遗传变化，尤其是与追踪随机序列的类似技术一起使用时。
