抗人纤维蛋白原特异性抗体群体的分离与鉴定
Isolation and characterization of a specific antibody population against human fibrinogen.
作者信息
Lau H K, Cheung M, Siu A
机构信息
Department of Biochemistry, University of Hong Kong.
出版信息
Biochem Int. 1987 Sep;15(3):561-9.
A specific antibody population against human fibrinogen was isolated from a rabbit antiserum by affinity chromatography on fibrinogen-bound Sepharose gel. Using a sensitive competitive radioimmunoassay, the antibody population was found to recognize epitopes on native fibrinogen but crossreacted minimally with fibrinogen fragment D and an early plasmin-degraded fibrinogen A alpha-chain product, but not at all with fragment E or fibrinopeptides A and B. Fibrin monomers shared part of these epitopes. The antibody population crossreacted to a small extent with bovine, horse and baboon fibrinogens and not at all with fibrinogens from sheep, rat, pig, goat, guinea pig, dog and rabbit.
通过在结合纤维蛋白原的琼脂糖凝胶上进行亲和层析，从兔抗血清中分离出了针对人纤维蛋白原的特异性抗体群体。使用灵敏的竞争性放射免疫测定法，发现该抗体群体可识别天然纤维蛋白原上的表位，但与纤维蛋白原片段D和早期纤溶酶降解的纤维蛋白原Aα链产物的交叉反应极小，而与片段E或纤维蛋白肽A和B则完全不发生交叉反应。纤维蛋白单体共享这些表位的一部分。该抗体群体与牛、马和狒狒的纤维蛋白原存在小程度的交叉反应，而与绵羊、大鼠、猪、山羊、豚鼠、狗和兔的纤维蛋白原则完全不发生交叉反应。
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