Joeng Kyu Sang, Regan Jenna, Long Fanxin
Baylor College of Medicine, Houston, TX, USA.
Washington University School of Medicine, St. Louis, MO, USA.
Methods Mol Biol. 2014;1130:217-232. doi: 10.1007/978-1-62703-989-5_16.
In situ hybridization (ISH) using RNA probes is a valuable technique to characterize gene expression patterns in animal tissues. It provides valuable spatial information about gene expression. Compared to the nonradioactive alternatives,(35)S radioactive ISH generally provides higher sensitivity. Here, we describe the procedure for(35)S ISH on paraffin sections from the skeletal tissues of mouse embryos.
使用RNA探针的原位杂交（ISH）是一种用于表征动物组织中基因表达模式的重要技术。它提供了有关基因表达的有价值的空间信息。与非放射性替代方法相比，（35）S放射性ISH通常具有更高的灵敏度。在这里，我们描述了对小鼠胚胎骨骼组织石蜡切片进行（35）S ISH的方法。
