放射性原位杂交检测骨骼组织切片中的基因表达。

Radioactive in situ hybridization to detect gene expression in skeletal tissue sections.

作者信息

Joeng Kyu Sang, Regan Jenna, Long Fanxin

机构信息

Baylor College of Medicine, Houston, TX, USA.

Washington University School of Medicine, St. Louis, MO, USA.

出版信息

Methods Mol Biol. 2014;1130:217-232. doi: 10.1007/978-1-62703-989-5_16.

DOI:10.1007/978-1-62703-989-5_16

PMID:24482176

Abstract

In situ hybridization (ISH) using RNA probes is a valuable technique to characterize gene expression patterns in animal tissues. It provides valuable spatial information about gene expression. Compared to the nonradioactive alternatives,(35)S radioactive ISH generally provides higher sensitivity. Here, we describe the procedure for(35)S ISH on paraffin sections from the skeletal tissues of mouse embryos.

摘要

使用RNA探针的原位杂交（ISH）是一种用于表征动物组织中基因表达模式的重要技术。它提供了有关基因表达的有价值的空间信息。与非放射性替代方法相比，（35）S放射性ISH通常具有更高的灵敏度。在这里，我们描述了对小鼠胚胎骨骼组织石蜡切片进行（35）S ISH的方法。