Shwartz Yulia, Zelzer Elazar
Weizmann Institute of Science, Rehovot, Israel.
Methods Mol Biol. 2014;1130:203-215. doi: 10.1007/978-1-62703-989-5_15.
Spatial and temporal visualization of RNA transcripts in tissue is a key tool in studying both developmental and pathological processes. In situ hybridization is a highly sensitive method for RNA transcript detection. It is based on sequence complementation between a labeled RNA probe and the RNA transcript of interest. The labeled probe is then detected by immunohistochemical methods using an antibody conjugated to an enzyme that catalyzes the generation of chromogenic or fluorescent signals, which indicate the location of the transcript.
组织中RNA转录本的时空可视化是研究发育和病理过程的关键工具。原位杂交是一种用于RNA转录本检测的高度灵敏的方法。它基于标记的RNA探针与目标RNA转录本之间的序列互补性。然后通过免疫组织化学方法，使用与催化生色或荧光信号产生的酶偶联的抗体来检测标记的探针，这些信号指示转录本的位置。
