Horticulture Department, Purdue University, Lafayette, Indiana.
Planta. 1972 Sep;102(3):179-89. doi: 10.1007/BF00386889.
Gamma-irradiation dosages between 100 and 200 krad greatly stimulate the development of invertase activity in sugar-beet tissue. However, exposure of tissue to 800 krad virtually eliminates the production of invertase. The production of the enzyme in control and irradiated tissue requires RNA and protein synthesis. Failure of 5-fluorouracil to inhibit the development of invertase implies that the synthesis of ribosomal RNA is not required for enzyme production. A close correlation between irradiation-stimulated methylation of sRNA and enzyme production is noted. We suggest that the synthesis or modification of some RNA required for the translation of "masked invertase RNA" is stimulated by γ-irradiation.
伽马射线照射剂量在 100 到 200 千拉德之间会极大地刺激甜菜组织中转化酶活性的发展。然而,组织暴露在 800 千拉德下几乎会消除转化酶的产生。对照和辐照组织中酶的产生需要 RNA 和蛋白质合成。5-氟尿嘧啶未能抑制转化酶的产生表明核糖体 RNA 的合成不是酶产生所必需的。我们注意到,辐照刺激 sRNA 甲基化与酶产生之间存在密切相关性。我们建议,用于翻译“掩蔽的转化酶 RNA”的某些 RNA 的合成或修饰受到 γ 射线照射的刺激。