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A simple ELISA for the classification of monoclonal antibodies according to their recognition of native epitopes.

作者信息

Kenett D

机构信息

Department of Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel.

出版信息

J Immunol Methods. 1988 Feb 10;106(2):203-9. doi: 10.1016/0022-1759(88)90198-6.

DOI:10.1016/0022-1759(88)90198-6
PMID:2448386
Abstract

A sandwich ELISA for testing whether pairs of monoclonal antibodies recognize the same native antigenic site was developed. The assay was performed with murine anti-lysozyme monoclonal antibodies. A monoclonal antibody, adsorbed onto a microtiter plate, was used as a capture antibody for native lysozyme. After the reaction with the antigen a second monoclonal antibody, the test antibody, was added. The amount of bound antibody was quantitatively measured using rabbit anti-mouse immunoglobulin serum conjugated to alkaline phosphatase. The simultaneous binding of pairs of monoclonal antibodies to lysozyme was further substantiated by structural considerations.

摘要

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