单克隆抗体和多特异性抗体在IgE夹心酶联免疫吸附测定中的应用。

The use of monoclonal and polyspecific antibodies in the IgE sandwich ELISA.

作者信息

Kemeny D M, Urbanek R, Samuel D, Richards D, Maasch H J

出版信息

J Immunol Methods. 1986 Feb 27;87(1):45-50. doi: 10.1016/0022-1759(86)90342-x.

Abstract

The use of antibody to link antigen to microtitre plates in the enzyme-linked immunosorbent assay (ELISA) has been extended to include mouse monoclonal antibodies and polyspecific rabbit antibodies. Small amounts of both reagents could be used. The capacity of the microtitre plate for the antibody was found to be critical and irradiated plates generally gave better results. Both rabbit anti-IgE conjugated to horseradish peroxidase, and monoclonal mouse anti-IgE with alkaline phosphatase-conjugated rabbit anti-mouse IgG could be used as detection reagents. Comparison with the radioallergosorbent (RAST) test showed a good agreement with the sandwich ELISA. The sandwich ELISA using polyspecific rabbit antibody was substantially more sensitive than conventional ELISA and also marginally more sensitive than RAST.

摘要

在酶联免疫吸附测定（ELISA）中，使用抗体将抗原连接到微量滴定板的方法已扩展到包括小鼠单克隆抗体和多特异性兔抗体。两种试剂都可以使用少量。发现微量滴定板对抗体的容量至关重要，辐照过的板通常能产生更好的结果。与辣根过氧化物酶结合的兔抗IgE以及与碱性磷酸酶结合的兔抗小鼠IgG的小鼠抗IgE单克隆抗体都可以用作检测试剂。与放射变应原吸附（RAST）试验的比较表明，夹心ELISA与之吻合良好。使用多特异性兔抗体的夹心ELISA比传统ELISA灵敏度大幅提高，也比RAST略敏感。