Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund, Germany.
Physikalische Chemie, Fakultät für Chemie und Chemische Biologie, TU Dortmund, Otto-Hahn Strasse 6, 44227 Dortmund, Germany.
Phys Rev Lett. 2014 Jan 17;112(2):028101. doi: 10.1103/PhysRevLett.112.028101. Epub 2014 Jan 14.
We present results from small-angle x-ray scattering data on the effect of high pressure on the phase behavior of dense lysozyme solutions in the liquid-liquid phase separation region, and characterize the underlying intermolecular protein-protein interactions as a function of temperature and pressure in this region of phase space. A reentrant liquid-liquid phase separation region has been discovered at elevated pressures, which originates in the pressure dependence of the solvent-mediated protein-protein interactions.
我们展示了小角度 X 射线散射数据的结果，这些结果表明高压对浓溶菌酶溶液在液-液相分离区域的相行为的影响，并在该相空间区域中作为温度和压力的函数来描述潜在的分子间蛋白质-蛋白质相互作用。在升高的压力下发现了一个再进入的液-液相分离区域，其起源于溶剂介导的蛋白质-蛋白质相互作用的压力依赖性。
