Yoshimura Aya, Koketsu Mami, Bando Hironori, Saiki Erisha, Suzuki Moemi, Watanabe Yusaku, Kanuka Hirotaka, Fukumoto Shinya
1 National Research Center of Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
J Wildl Dis. 2014 Apr;50(2):235-42. doi: 10.7589/2013-03-071. Epub 2014 Jan 31.
We analyzed blood samples of resident and migratory Japanese birds to evaluate the prevalence and genetic background of avian blood parasites in northern Japan. We used PCR targeting the mitochondrial cytochrome b gene to examine infections of Leucocytozoon, Haemoproteus, and Plasmodium parasites in blood samples from 243 birds of 14 species in three orders (Passeriformes, Columbiformes, and Anseriformes). Sequences were subjected to phylogenetic analysis. The infection rate was 21% in pigeons (Columbiformes) and 17% in Anseriformes. A high infection rate of 93.8% was found in crow species (Passeriformes). Haemoproteus and Plasmodium parasites were detected in only two species. Infected blood samples obtained from seven bird species involved two major clades of Leucocytozoon, which were divided between resident and migratory birds. The parasites, which are genetically distinct from parasites in Japanese resident birds, may have been introduced to Japan by migratory bird species.
我们分析了日本留鸟和候鸟的血液样本，以评估日本北部鸟类血液寄生虫的流行情况和遗传背景。我们使用针对线粒体细胞色素b基因的聚合酶链反应（PCR），检测了来自三个目（雀形目、鸽形目和雁形目）14种243只鸟类血液样本中的住白细胞原虫、血变原虫和疟原虫感染情况。对序列进行了系统发育分析。鸽形目的感染率为21%，雁形目的感染率为17%。在雀形目的乌鸦种类中发现了高达93.8%的高感染率。仅在两个物种中检测到血变原虫和疟原虫。从七种鸟类获得的感染血液样本涉及住白细胞原虫的两个主要进化枝，它们在留鸟和候鸟之间分布。这些寄生虫在基因上与日本留鸟中的寄生虫不同，可能是由候鸟引入日本的。
