Abbott Rachel C, Hudak Robert, Mondesire Roy, Baeten Laurie A, Russell Robin E, Rocke Tonie E
1 USGS National Wildlife Health Center, 6006 Schroeder Rd., Madison, Wisconsin 53711, USA.
J Wildl Dis. 2014 Apr;50(2):384-8. doi: 10.7589/2013-07-174. Epub 2014 Jan 31.
Plague surveillance is routinely conducted to predict future epizootics in wildlife and exposure risk for humans. The most common surveillance method for sylvatic plague is detection of antibodies to Yersinia pestis F1 capsular antigen in sentinel animals, such as coyotes (Canis latrans). Current serologic tests for Y. pestis, hemagglutination (HA) test and enzyme-linked immunosorbent assay (ELISA), are expensive and labor intensive. To address this need, we developed a complete lateral flow device for the detection of specific antibodies to Y. pestis F1 and V antigens. Our test detected anti-F1 and anti-V antibodies in serum and Nobuto filter paper samples from coyotes, and in serum samples from prairie dogs (Cynomys ludovicianus), lynx (Lynx canadensis), and black-footed ferrets (Mustela nigripes). Comparison of cassette results for anti-F1 and anti-V antibodies with results of ELISA or HA tests showed correlations ranging from 0.68 to 0.98. This device provides an affordable, user-friendly tool that may be useful in plague surveillance programs and as a research tool.
定期开展鼠疫监测，以预测野生动物未来的 epizootics 以及人类的暴露风险。对腺鼠疫最常见的监测方法是在诸如郊狼（犬属）等哨兵动物中检测鼠疫耶尔森菌 F1 荚膜抗原的抗体。目前用于鼠疫耶尔森菌的血清学检测，即血凝（HA）试验和酶联免疫吸附测定（ELISA），成本高且劳动强度大。为满足这一需求，我们开发了一种完整的侧向流动装置，用于检测鼠疫耶尔森菌 F1 和 V 抗原的特异性抗体。我们的检测在郊狼的血清和野口滤纸样本中，以及在草原犬鼠（黑尾草原犬鼠）、猞猁（加拿大猞猁）和黑足雪貂（黑足鼬）的血清样本中检测到了抗 F1 和抗 V 抗体。将抗 F1 和抗 V 抗体的检测盒结果与 ELISA 或 HA 试验结果进行比较，显示相关性在 0.68 至 0.98 之间。该装置提供了一种经济实惠、用户友好的工具，可能在鼠疫监测计划中有用，并可作为一种研究工具。
