Shi-Xiao Jin, Shi-Ying Jin, Lv Qing-Yuan, Shan-Shan Fu, Jin Han, Yuan Hai-Long
Department of Pharmacy, 302 Military Hospital, Beijing 100039, China; School of pharmaceutics, Chengdu University of TCM, Chengdu 611730, China.
Department of Pharmacy, 302 Military Hospital, Beijing 100039, China.
Chin J Nat Med. 2014 Jan;12(1):65-70. doi: 10.1016/S1875-5364(14)60011-X.
To improve the absorption of thymopeptides (TH) by preparing sodium deoxycholate/phospholipid-mixed nanomicelles (SDC/PL-MMs).
TH-SDC/PL-MMs were prepared by a film dispersion method, and then evaluated using photon correlation spectroscopy (PCS), zeta potential measurement, as well as their physical stability after storage for several days. Furthermore, in situ intestinal single-pass perfusion experiments and pharmacodynamics in immunodeficient mice were performed to make a comparison with TH powders and the control drug in absorption properties.
A narrow size distribution of nanomicelles, with a mean particle size of (149 ± 8.32) nm and a zeta potential of (-31.05 ± 2.52) mV, was obtained. The in situ intestine perfusion experiments demonstrated a significant advantage in absorption characteristics for TH compared to the other formulations, and oral administration of TH-SDC/PL-MMs potentiated an equivalent effect with i.h. TH in pharmacodynamic studies in immunodeficient mice.
TH-SDC/PL-MMs prepared by a film dispersion method are able to improve the absorption of TH. SDC/PL-MMs might be a good approach for the more effective delivery of drugs like TH.
通过制备脱氧胆酸钠/磷脂混合纳米胶束（SDC/PL-MMs）来提高胸腺肽（TH）的吸收。
采用薄膜分散法制备TH-SDC/PL-MMs，然后用光子相关光谱法（PCS）、ζ电位测量法对其进行评估，并观察其储存数天后的物理稳定性。此外，进行了原位肠道单通道灌注实验和免疫缺陷小鼠的药效学实验，以比较TH粉末和对照药物的吸收特性。
获得了纳米胶束窄尺寸分布，平均粒径为（149±8.32）nm，ζ电位为（-31.05±2.52）mV。原位肠道灌注实验表明，与其他制剂相比，TH在吸收特性方面具有显著优势，并且在免疫缺陷小鼠的药效学研究中，口服TH-SDC/PL-MMs与皮下注射TH具有等效效果。
通过薄膜分散法制备的TH-SDC/PL-MMs能够提高TH的吸收。SDC/PL-MMs可能是更有效地递送如TH这类药物的良好方法。
