• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人卵巢卵泡发育过程中基质金属蛋白酶-14、基质金属蛋白酶-2的免疫组化表达及基质金属蛋白酶-2活性

Immunohistochemical expression of MMP-14 and MMP-2, and MMP-2 activity during human ovarian follicular development.

作者信息

Vos Maria Caroline, van der Wurff Anneke Am, Last Jessie T J, de Boed Ella A M, Smeenk Jesper M J, van Kuppevelt Toin H, Massuger Leon F A G

机构信息

Department of Obstetrics and Gynaecology, St, Elisabeth Hospital, PO Box 90151, 5000 LC Tilburg, the Netherlands.

出版信息

Reprod Biol Endocrinol. 2014 Jan 31;12:12. doi: 10.1186/1477-7827-12-12.

DOI:10.1186/1477-7827-12-12
PMID:24485069
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3937151/
Abstract

BACKGROUND

The aim of this study was to investigate the presence of MMP-14 and MMP-2 during human ovarian follicular development using immunohistochemistry, and the activity of MMP-2 in follicular fluid using zymography.

METHODS

Ovarian tissue collected from the archives of the Department of Pathology was examined and medical records and histopathology were reviewed. Follicular fluids were collected at the IVF-department and analyzed using zymography.

RESULTS

MMP-14 and MMP-2 were increasingly found in the growing follicles and MMP-2 was highly expressed in the corpus luteum. Pro-MMP-2 was present in follicular fluid of IVF-patients.

CONCLUSIONS

The presence of MMP-14 and MMP-2 during human ovarian follicular development from the primordial follicle to the tertiary follicle and corpus luteum is confirmed, as was indicated by earlier animal studies following stimulation with gonadotrophins.

摘要

背景

本研究旨在采用免疫组织化学方法调查人卵巢卵泡发育过程中基质金属蛋白酶-14（MMP-14）和基质金属蛋白酶-2（MMP-2）的存在情况，并采用酶谱分析法检测卵泡液中MMP-2的活性。

方法

检查从病理科档案中收集的卵巢组织，并查阅病历和组织病理学资料。在体外受精科收集卵泡液，并采用酶谱分析法进行分析。

结果

在生长卵泡中越来越多地发现MMP-14和MMP-2，且MMP-2在黄体中高表达。原MMP-2存在于体外受精患者的卵泡液中。

结论

正如早期用促性腺激素刺激后的动物研究所示，证实了从原始卵泡到三级卵泡及黄体的人卵巢卵泡发育过程中存在MMP-14和MMP-2。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/c9bf7e083c77/1477-7827-12-12-13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/3e4021c371d0/1477-7827-12-12-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/ddc0ef8b3ec6/1477-7827-12-12-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/f993caa76540/1477-7827-12-12-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/caa6259da854/1477-7827-12-12-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/301b7e3b7c07/1477-7827-12-12-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/aa0c5c6f2eec/1477-7827-12-12-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/8184e7a664ce/1477-7827-12-12-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/9f308b8c6b51/1477-7827-12-12-8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/828dfeec08a4/1477-7827-12-12-9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/5c4ca030358d/1477-7827-12-12-10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/82e67684b09a/1477-7827-12-12-11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/e2d7ee96715d/1477-7827-12-12-12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/c9bf7e083c77/1477-7827-12-12-13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/3e4021c371d0/1477-7827-12-12-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/ddc0ef8b3ec6/1477-7827-12-12-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/f993caa76540/1477-7827-12-12-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/caa6259da854/1477-7827-12-12-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/301b7e3b7c07/1477-7827-12-12-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/aa0c5c6f2eec/1477-7827-12-12-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/8184e7a664ce/1477-7827-12-12-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/9f308b8c6b51/1477-7827-12-12-8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/828dfeec08a4/1477-7827-12-12-9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/5c4ca030358d/1477-7827-12-12-10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/82e67684b09a/1477-7827-12-12-11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/e2d7ee96715d/1477-7827-12-12-12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d373/3937151/c9bf7e083c77/1477-7827-12-12-13.jpg

相似文献

1
Immunohistochemical expression of MMP-14 and MMP-2, and MMP-2 activity during human ovarian follicular development.人卵巢卵泡发育过程中基质金属蛋白酶-14、基质金属蛋白酶-2的免疫组化表达及基质金属蛋白酶-2活性
Reprod Biol Endocrinol. 2014 Jan 31;12:12. doi: 10.1186/1477-7827-12-12.
2
Secretion of matrix metalloproteinases 2 and 9 and tissue inhibitor of metalloproteinases into follicular fluid during follicle development in equine ovaries.马卵巢卵泡发育过程中基质金属蛋白酶2和9及金属蛋白酶组织抑制剂向卵泡液中的分泌。
Reproduction. 2001 Apr;121(4):553-60.
3
Matrix metalloproteinase-2 and -9 secretion by the equine ovary during follicular growth and prior to ovulation.卵泡生长期间及排卵前马卵巢中基质金属蛋白酶-2和-9的分泌
Anim Reprod Sci. 2004 Apr;81(3-4):329-39. doi: 10.1016/j.anireprosci.2003.10.011.
4
Decreased expression of tissue inhibitor of matrix metalloproteinases in follicular fluid from women with polycystic ovaries compared with normally ovulating patients undergoing in vitro fertilization.与接受体外受精的正常排卵患者相比，多囊卵巢女性卵泡液中基质金属蛋白酶组织抑制剂的表达降低。
Fertil Steril. 2003 Mar;79(3):567-71. doi: 10.1016/s0015-0282(02)04838-0.
5
Matrix metalloproteinases-2 and -9 activities in bovine follicular fluid of different-sized follicles: relationship to intra-follicular inhibin and steroid concentrations.不同大小卵泡的牛卵泡液中基质金属蛋白酶-2和-9的活性：与卵泡内抑制素和类固醇浓度的关系。
Domest Anim Endocrinol. 2003 Mar;24(2):171-83. doi: 10.1016/s0739-7240(02)00235-7.
6
Abundant expression of sodium-potassium-activated adenosinetriphosphatase alpha 1 subunit in corpus luteum of porcine ovary.钠钾激活的三磷酸腺苷酶α1亚基在猪卵巢黄体中的大量表达。
Endocrine. 2003 Aug;21(3):233-40. doi: 10.1385/ENDO:21:3:233.
7
Cellular localization of gelatinases and tissue inhibitors of metalloproteinases during follicular growth, ovulation, and early luteal formation in the rat.大鼠卵泡生长、排卵及早期黄体形成过程中明胶酶和金属蛋白酶组织抑制剂的细胞定位
Biol Reprod. 2001 Sep;65(3):855-65. doi: 10.1095/biolreprod65.3.855.
8
Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles.人卵泡液中基质金属蛋白酶2水平是体外受精和卵胞浆内单精子注射周期中人卵母细胞成熟的可靠标志物。
Reprod Biol Endocrinol. 2015 Sep 4;13:102. doi: 10.1186/s12958-015-0099-8.
9
Gene expression of matrix metalloproteinases and LH receptors in mare follicular development.基质金属蛋白酶和促黄体生成素受体在母马卵泡发育中的基因表达
Theriogenology. 2014 Nov;82(8):1131-6. doi: 10.1016/j.theriogenology.2014.07.033. Epub 2014 Aug 1.
10
Evaluation of matrix metalloproteinases-1 and -3 concentrations in the tunica albuginea, the apical wall of atretic follicles and the corpus luteum of normal human ovaries.正常人类卵巢白膜、闭锁卵泡顶壁及黄体中基质金属蛋白酶-1和-3浓度的评估。
Gynecol Endocrinol. 2000 Feb;14(1):25-31. doi: 10.3109/09513590009167656.

引用本文的文献

1
Spotlight on Proteases: Roles in Ovarian Health and Disease.蛋白酶聚焦：在卵巢健康与疾病中的作用
Cells. 2025 Jun 18;14(12):921. doi: 10.3390/cells14120921.
2
Molecular evidence for pre-chordate origins of ovarian cell types and neuroendocrine control of reproduction.卵巢细胞类型的前脊索动物起源及生殖的神经内分泌控制的分子证据。
bioRxiv. 2025 Mar 28:2025.03.24.644836. doi: 10.1101/2025.03.24.644836.
3
Proteomics profiling reveals lipid metabolism abnormalities during oogenesis in unexplained recurrent pregnancy loss.蛋白质组学分析揭示不明原因复发性妊娠丢失中卵母细胞发生过程中的脂质代谢异常。

本文引用的文献

1
Physical limits of cell migration: control by ECM space and nuclear deformation and tuning by proteolysis and traction force.细胞迁移的物理限制：细胞外基质空间和核变形的控制，以及蛋白水解和牵引力的调整。
J Cell Biol. 2013 Jun 24;201(7):1069-84. doi: 10.1083/jcb.201210152.
2
The extracellular matrix remodeled: Interdependency of matrix proteolysis, cell adhesion, and force sensing.细胞外基质重塑：基质蛋白水解、细胞黏附与力感知的相互依赖性。
Commun Integr Biol. 2012 Jan 1;5(1):71-3. doi: 10.4161/cib.17342.
3
Matrix metalloproteinases 2 and 9 and their tissue inhibitors in the follicular fluid of patients with polycystic ovaries undergoing in vitro fertilisation.
Front Immunol. 2024 Aug 8;15:1397633. doi: 10.3389/fimmu.2024.1397633. eCollection 2024.
4
Comparative Tensile Properties and Collagen Patterns in Domestic Cat () and Dog () Ovarian Cortical Tissues.家猫（ ）和家犬（ ）卵巢皮质组织的拉伸性能及胶原蛋白模式比较
Bioengineering (Basel). 2023 Nov 4;10(11):1285. doi: 10.3390/bioengineering10111285.
5
Utility of Matrix Metalloproteinases in the Diagnosis, Monitoring and Prognosis of Ovarian Cancer Patients.基质金属蛋白酶在卵巢癌患者诊断、监测及预后中的应用价值
Cancer Manag Res. 2022 Nov 30;14:3359-3382. doi: 10.2147/CMAR.S385658. eCollection 2022.
6
Metalloproteases in gonad formation and ovulation.金属蛋白酶在性腺形成和排卵中的作用。
Gen Comp Endocrinol. 2021 Dec 1;314:113924. doi: 10.1016/j.ygcen.2021.113924. Epub 2021 Oct 2.
7
Downregulation of Extracellular Matrix and Cell Adhesion Molecules in Cumulus Cells of Infertile Polycystic Ovary Syndrome Women with and without Insulin Resistance.伴有和不伴有胰岛素抵抗的多囊卵巢综合征不孕女性卵丘细胞中细胞外基质和细胞粘附分子的下调
Cell J. 2019 Jan;21(1):35-42. doi: 10.22074/cellj.2019.5576. Epub 2018 Nov 18.
8
Developmental Programming: Gestational Exposure to Excess Testosterone Alters Expression of Ovarian Matrix Metalloproteases and Their Target Proteins.发育编程：孕期暴露于过量睾酮会改变卵巢基质金属蛋白酶及其靶蛋白的表达。
Reprod Sci. 2018 Jun;25(6):882-892. doi: 10.1177/1933719117697127. Epub 2017 Mar 16.
9
Gelatin Binding Proteins in Reproductive Physiology.生殖生理学中的明胶结合蛋白
Indian J Microbiol. 2016 Dec;56(4):383-393. doi: 10.1007/s12088-016-0618-0. Epub 2016 Sep 16.
10
MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer.基质金属蛋白酶-14和CD44在卵巢癌上皮-间质转化（EMT）中的作用
J Ovarian Res. 2016 Sep 2;9(1):53. doi: 10.1186/s13048-016-0262-7.
多囊卵巢患者体外受精中卵泡液中的基质金属蛋白酶 2 和 9 及其组织抑制剂。
In Vivo. 2010 May-Jun;24(3):293-6.
4
Ovarian furin (proprotein convertase subtilisin/kexin type3): expression, localization, and potential role in ovulation in the rat.卵巢弗林蛋白酶（蛋白原转化酶枯草溶菌素/柯萨奇病毒蛋白酶 3）：在大鼠排卵中的表达、定位和潜在作用。
Biol Reprod. 2010 Jul;83(1):147-54. doi: 10.1095/biolreprod.109.079947. Epub 2010 Apr 7.
5
Functional corpora lutea are formed in matrix metalloproteinase inhibitor-treated plasminogen-deficient mice.功能性黄体在基质金属蛋白酶抑制剂处理的纤溶酶原缺陷小鼠中形成。
Endocrinology. 2007 Mar;148(3):1226-34. doi: 10.1210/en.2006-0669. Epub 2006 Nov 22.
6
Gelatinases and their tissue inhibitors during human ovulation: increased expression of tissue inhibitor of matrix metalloproteinase-1.人类排卵过程中的明胶酶及其组织抑制剂：基质金属蛋白酶-1组织抑制剂表达增加。
Mol Hum Reprod. 2006 Dec;12(12):725-36. doi: 10.1093/molehr/gal086. Epub 2006 Oct 27.
7
Circulating gelatinases and tissue inhibitor of metalloproteinase-1 in colorectal cancer metastatic liver disease.循环明胶酶与金属蛋白酶组织抑制因子-1在结直肠癌肝转移疾病中的作用
Eur J Surg Oncol. 2006 Sep;32(7):756-63. doi: 10.1016/j.ejso.2006.03.028. Epub 2006 May 2.
8
The clinical relevance of stromal matrix metalloproteinase expression in ovarian cancer.基质金属蛋白酶在卵巢癌中的表达的临床相关性。
Clin Cancer Res. 2006 Mar 15;12(6):1707-14. doi: 10.1158/1078-0432.CCR-05-2338.
9
Zymographic techniques for the analysis of matrix metalloproteinases and their inhibitors.用于分析基质金属蛋白酶及其抑制剂的酶谱技术。
Biotechniques. 2005 Jan;38(1):73-83. doi: 10.2144/05381RV01.
10
MMPS and TIMPS in ovarian physiology and pathophysiology.基质金属蛋白酶和金属蛋白酶组织抑制因子在卵巢生理与病理生理中的作用
Front Biosci. 2004 Sep 1;9:2474-83. doi: 10.2741/1409.