Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Rua Professor Mário Werneck S/N°, Cidade Universitária - Campus da UFMG, 31120-970, Belo Horizonte, MG, Brazil.
Diagn Microbiol Infect Dis. 2014 Apr;78(4):418-21. doi: 10.1016/j.diagmicrobio.2013.10.015. Epub 2013 Oct 31.
The canine visceral leishmaniasis (CVL) diagnosis is an important step of visceral leishmaniasis control program in Brazil, which involves the elimination of infected dogs, the main animal reservoir host of the disease. The aim of the present study was to evaluate a sensitive real-time PCR method for Leishmania infantum detection in 4 different clinical samples of dogs, including the noninvasive conjunctival swab (CS) sample. The results of real-time PCR were compared with those obtained using internal transcribed spacer 1 nested PCR. Animals were divided into 2 groups based on the absence or presence of CVL clinical sings. The CS associated with real-time PCR, using primers addressed to kinetoplast DNA minicircles, was able to detect L. infantum infection in 96.7% of dogs without clinical signs and in 100% of the symptomatic animals, demonstrating the importance of these procedures for diagnosing CVL.
犬内脏利什曼病（CVL）的诊断是巴西内脏利什曼病控制计划的重要步骤，其中包括消除受感染的犬，这是该病的主要动物储存宿主。本研究的目的是评估一种敏感的实时 PCR 方法，用于检测 4 种不同的犬临床样本中的利什曼原虫，包括非侵入性的结膜拭子（CS）样本。实时 PCR 的结果与使用内部转录间隔区 1 嵌套 PCR 获得的结果进行了比较。根据是否存在 CVL 临床症状，将动物分为 2 组。使用针对动基体 DNA 微环的引物的 CS 与实时 PCR 相关联，能够在 96.7%无临床症状的犬和 100%的有症状动物中检测到 L. infantum 感染，这表明这些程序对于诊断 CVL 非常重要。
Diagn Microbiol Infect Dis. 2013-10-31
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