Strauss-Ayali Dalit, Jaffe Charles L, Burshtain Ofer, Gonen Liat, Baneth Gad
School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel.
J Infect Dis. 2004 May 1;189(9):1729-33. doi: 10.1086/383281. Epub 2004 Apr 13.
A polymerase chain reaction (PCR) procedure using noninvasively obtained samples, for the identification of Leishmania infantum in canine tissues, was evaluated and compared with serologic testing and culture. A total of 92% of naturally infected, symptomatic, seropositive dogs were found to be positive by use of DNA from conjunctival swabs. Spleen or lymph node aspirates were found to be positive by PCR in 86% and by culture in 74% of these dogs. The sensitivity and specificity of conjunctival PCR were 92% and 100%, respectively. Experimentally infected dogs were found to be positive by conjunctival PCR already at 45 days of infection (83%) and before seroconversion. PCR using noninvasively obtained conjunctival samples will be useful for epidemiological studies and for direct diagnosis of canine visceral leishmaniasis.
对一种使用非侵入性获取样本的聚合酶链反应（PCR）程序进行了评估，该程序用于鉴定犬类组织中的婴儿利什曼原虫，并与血清学检测和培养进行了比较。通过使用结膜拭子的DNA，发现92%自然感染、有症状且血清学阳性的犬呈阳性。在这些犬中，脾脏或淋巴结穿刺液经PCR检测阳性率为86%，培养检测阳性率为74%。结膜PCR的敏感性和特异性分别为92%和100%。实验感染的犬在感染45天时（83%）以及血清转化前经结膜PCR检测呈阳性。使用非侵入性获取的结膜样本进行PCR将有助于流行病学研究和犬内脏利什曼病的直接诊断。
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