根据角蛋白的结构同源性及其组织特异性表达筛选出的编码人角蛋白10的cDNA序列。
Sequence of a cDNA encoding human keratin No 10 selected according to structural homologies of keratins and their tissue-specific expression.
作者信息
Darmon M Y, Sémat A, Darmon M C, Vasseur M
机构信息
Centre International de Recherches Dermatologiques (CIRD), Sophia Antipolis, Valbonne, France.
出版信息
Mol Biol Rep. 1987;12(4):277-83. doi: 10.1007/BF00444680.
We present here the nucleotide sequence of a 1700 bp-long cDNA encoding human epidermal keratin No. 10 (56.5 kDa). cDNA clones of the acidic keratin family were first isolated from a pBR322 human epidermal cDNA library by hybridization with a probe coding for keratin No. 14. Differential hybridization using total cDNA probes prepared from poly(A)+ RNA extracted either from epidermis (which contains keratin No. 10) and from squamous carcinoma or hepatoma cell lines (which do not express keratin No. 10) made possible the selection of clones potentially coding for keratin No. 10. The 1.7 kb sequence exhibits the characteristic features of an acidic keratin with a constant central rod domain and C-terminal variable structures. Moreover, the sequence shows extensive homologies with the cDNA of murine keratin No. 10.
我们在此展示了一段1700 bp长的编码人表皮角蛋白10（56.5 kDa）的cDNA的核苷酸序列。酸性角蛋白家族的cDNA克隆最初是通过与编码角蛋白14的探针杂交，从pBR322人表皮cDNA文库中分离出来的。使用从表皮（含角蛋白10）以及鳞状癌细胞或肝癌细胞系（不表达角蛋白10）提取的poly(A)+ RNA制备的总cDNA探针进行差异杂交，使得选择可能编码角蛋白10的克隆成为可能。该1.7 kb序列展现出酸性角蛋白的特征，具有恒定的中央杆状结构域和C端可变结构。此外，该序列与小鼠角蛋白10的cDNA具有广泛的同源性。
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