Tseng S C, Jarvinen M J, Nelson W G, Huang J W, Woodcock-Mitchell J, Sun T T
Cell. 1982 Sep;30(2):361-72. doi: 10.1016/0092-8674(82)90234-3.
We have prepared three monoclonal antibodies against human epidermal keratins. These antibodies were highly specific for keratins and, in combination, recognized all major epidermal keratins of several mammalian species. We have used these antibodies to study the tissue distribution of epidermis-related keratins. In various mammalian epithelia, the antibodies recognized seven classes of keratins defined by their immunological reactivity and size. The 40, 46 and 52 kilodalton (kd) keratin classes were present in almost all epithelia; the 50 kd and 58 kd keratin classes were detected in all stratified squamous epithelia, but not in any simple epithelia; and the 56 kd and 65-67 kd keratin classes were unique to keratinized epidermis. Thus the expression of specific keratin classes appeared to correlate with different types of epithelial differentiation (simple versus stratified; keratinized versus nonkeratinized).
我们制备了三种针对人表皮角蛋白的单克隆抗体。这些抗体对角蛋白具有高度特异性，并且联合使用时能识别几种哺乳动物的所有主要表皮角蛋白。我们利用这些抗体来研究表皮相关角蛋白的组织分布。在各种哺乳动物上皮组织中，这些抗体识别出七类根据免疫反应性和大小定义的角蛋白。40、46和52千道尔顿（kd）的角蛋白类别几乎存在于所有上皮组织中；50 kd和58 kd的角蛋白类别在所有复层鳞状上皮中被检测到，但在任何单层上皮中均未检测到；而56 kd和65 - 67 kd的角蛋白类别是角质化表皮所特有的。因此，特定角蛋白类别的表达似乎与不同类型的上皮分化（单层与复层；角质化与非角质化）相关。
