Levy J B, Dorai T, Wang L H, Brugge J S
Department of Microbiology, State University of New York, Stony Brook 11794.
Mol Cell Biol. 1987 Nov;7(11):4142-5. doi: 10.1128/mcb.7.11.4142-4145.1987.
A cellular src (c-src) cDNA clone was isolated from a chicken embryonic brain cDNA library and characterized by DNA sequence analysis. Comparison with the published sequence of a chicken genomic c-src clone indicated that the brain cDNA clone contained an 18-base-pair insertion located between exons 3 and 4 of the c-src gene. The six amino acids encoded by the insertion caused an alteration in the electrophoretic mobility of the c-src gene product similar to that of the structurally distinct form of the src protein detected in neuronal cultures.
从鸡胚胎脑cDNA文库中分离出一个细胞源癌基因（c-src）cDNA克隆，并通过DNA序列分析对其进行了表征。与已发表的鸡基因组c-src克隆序列进行比较表明，脑cDNA克隆在c-src基因的外显子3和4之间含有一个18个碱基对的插入片段。该插入片段编码的六个氨基酸导致c-src基因产物的电泳迁移率发生改变，类似于在神经元培养物中检测到的结构不同形式的src蛋白。
