Yang Y F, Tan-ariya P, Sharma Y D, Kilejian A
Public Health Research Institute, New York, NY 10016.
Mol Biochem Parasitol. 1987 Nov;26(1-2):61-7. doi: 10.1016/0166-6851(87)90130-7.
A cDNA library constructed from ring-stage RNA isolated from Plasmodium falciparum FCR-3/Gambia was screened with immune human serum and two related positive clones were isolated. Nucleotide sequence analysis of these recombinant clones revealed an open translational reading frame for 681 amino acids with a calculated molecular weight of 74.3 kDa. The deduced amino acid sequence of the polypeptide shows extensive homology to several heat shock proteins (hsp) which have been described. Northern and Southern hybridization analysis indicates that P. falciparum has a second gene which shares common sequences with the hsp gene described in this study.
用免疫人血清筛选从恶性疟原虫FCR-3/冈比亚分离的环状期RNA构建的cDNA文库，分离出两个相关的阳性克隆。对这些重组克隆进行核苷酸序列分析，发现一个681个氨基酸的开放翻译阅读框，计算分子量为74.3 kDa。该多肽推导的氨基酸序列与已描述的几种热休克蛋白（hsp）具有广泛的同源性。Northern和Southern杂交分析表明，恶性疟原虫有第二个基因，与本研究中描述的hsp基因具有共同序列。
