Syin C, Goldman N D
Laboratory of Parasitic Biology and Biochemistry, Food and Drug Administration, Rockville, MD 20852-1448, USA.
Mol Biochem Parasitol. 1996 Jul;79(1):13-9. doi: 10.1016/0166-6851(96)02633-3.
We have identified a gene encoding the 60 kDa heat shock protein (hsp60) from a Plasmodium falciparum blood stage cDNA library. The deduced protein sequence encodes for a polypeptide of 577 amino acids with a calculated molecular weight of 62158 Da. The primary structure of P. falciparum hsp60 contains a putative mitochondrial targeting peptide at its amino-terminus and a GGM motif at its carboxyl-terminus. The overall structure exhibits strong conservation (approximately 50%) to the hsp60 from human and other eukaryotes, but only low homology (< 30%) to a recently reported P. falciparum chaperonin 60 gene. The P. falciparum hsp60 gene is located on chromosome 10. During heat shock, the level of hsp60 transcript in blood stage parasites increases significantly and its accumulation correlates with the duration of the induction.
我们从恶性疟原虫血液阶段cDNA文库中鉴定出一个编码60 kDa热休克蛋白（hsp60）的基因。推导的蛋白质序列编码一个577个氨基酸的多肽，计算分子量为62158 Da。恶性疟原虫hsp60的一级结构在其氨基末端含有一个推定的线粒体靶向肽，在其羧基末端含有一个GGM基序。整体结构与人类和其他真核生物的hsp60表现出很强的保守性（约50%），但与最近报道的恶性疟原虫伴侣蛋白60基因只有很低的同源性（<30%）。恶性疟原虫hsp60基因位于10号染色体上。在热休克期间，血液阶段寄生虫中hsp60转录本的水平显著增加，其积累与诱导持续时间相关。
