Polgar G, Zane L, Babbucci M, Barbisan F, Patarnello T, Rüber L, Papetti C
Department of Biology, Universiti Brunei Darussalam, Bandar Seri Begawan, Gadong, Negara, Brunei Darussalam; Institute of Ocean and Earth Science, University of Malaya, Kuala Lumpur, Malaysia.
Department of Biology, University of Padova, via G. Colombo 3, 35121 Padova, Italy.
Mol Phylogenet Evol. 2014 Apr;73:161-76. doi: 10.1016/j.ympev.2014.01.014. Epub 2014 Jan 31.
This study provides a first description of the phylogeographic patterns and evolutionary history of two species of the mudskipper genus Periophthalmus. These amphibious gobies are distributed throughout the whole Indo-Pacific region and Atlantic coast of Africa, in peritidal habitats of soft-bottom coastal ecosystems. Three sequence datasets of two widely distributed species, Periophthalmus argentilineatus and P. kalolo, were obtained by amplifying and sequencing two mtDNA markers (D-loop and 16S rDNA) and the nDNA rag1 region. The three datasets were then used to perform phylogeographic, demographic and population genetic analyses. Our results indicate that tectonic events and past climatic oscillations strongly contributed to shape present genetic differentiation, phylogeographic and demographic patterns. We found support for the monophyly of P. kalolo, and only shallow genetic differentiation between East-African and Indo-Malayan populations of this species. However, our collections of the morphospecies P. argentilineatus include three molecularly distinct lineages, one of them more closely related to P. kalolo. The presence of Miocenic timings for the most recent common ancestors of some of these morphologically similar clades, suggests the presence of strong stabilising selection in mudskippers' habitats. At population level, demographic analyses and palaeoecological records of mangrove ecosystems suggest that Pleistocene bottlenecks and expansion plus secondary contact events of the studied species were associated with recurrent sea transgressions during interglacials, and sea regressions or stable regimes during glacials, respectively.
本研究首次描述了弹涂鱼属（Periophthalmus）两种鱼类的系统地理学模式和进化历史。这些两栖虾虎鱼分布于整个印度-太平洋地区以及非洲大西洋沿岸，生活在软底沿海生态系统的潮间带栖息地。通过扩增和测序两个线粒体DNA标记（D-loop和16S rDNA）以及核DNA rag1区域，获得了两种广泛分布的物种——银线弹涂鱼（Periophthalmus argentilineatus）和大弹涂鱼（P. kalolo）的三个序列数据集。然后利用这三个数据集进行系统地理学、种群统计学和群体遗传学分析。我们的结果表明，构造事件和过去的气候振荡对当前的遗传分化、系统地理学和种群统计学模式的形成起到了重要作用。我们发现大弹涂鱼为单系群，且该物种的东非和印度-马来亚种群之间只有轻微的遗传分化。然而，我们收集的形态物种银线弹涂鱼包含三个分子上不同的谱系，其中一个与大弹涂鱼的关系更为密切。这些形态相似的分支中一些最近共同祖先的中新世时间，表明弹涂鱼栖息地存在强烈的稳定选择。在种群水平上，红树林生态系统的种群统计学分析和古生态记录表明，所研究物种的更新世瓶颈和扩张以及二次接触事件分别与间冰期的反复海侵以及冰期的海退或稳定状态有关。
