脱落酸、激动素和 5- 氟尿嘧啶对衰老萝卜叶圆片的核糖核酸和蛋白质合成的影响。
The effects of abscisic acid, kinetin and 5-fluorouracil on ribonucleic acid and protein synthesis in senescing radish leaf disks.
机构信息
Botany Department, University College of Wales, Aberystwyth.
出版信息
Planta. 1971 Jun;99(2):112-9. doi: 10.1007/BF00388243.
The effects of abscisic acid and kinetin on RNA synthesis in senescing radish leaf disks were investigated using the improved resolution afforded by polyacrylamide gel electrophoresis. Kinetin stimulated and abscisic acid inhibited incorporation of radioactivity into cytoplasmic ribosomal RNA and soluble RNA. Chloroplast ribosomal RNA synthesis appeared to be confined to the period of leaf expansion and was not detected in fully mature leaves. The effects of kinetin in retarding and of abscisic acid in accelerating leaf senescence were not altered by the inhibition of cytoplasmic ribosomal RNA synthesis with 5-fluorouracil. Following inhibition of cytoplasmic ribosomal RNA synthesis with 5-fluorouracil, kinetin stimulated and abscisic acid inhibited incorporation of radioactivity into polydisperse RNA. These results are discussed in relation to the possible mode of action of kinetin and abscisic acid in senescing leaf tissue.
用改进的聚丙烯酰胺凝胶电泳分辨率研究脱落酸和激动素对衰老萝卜叶圆片 RNA 合成的影响。激动素刺激并脱落酸抑制放射性掺入细胞质核糖体 RNA 和可溶性 RNA。叶绿体核糖体 RNA 的合成似乎仅限于叶片扩展期，在完全成熟的叶片中未检测到。用 5-氟尿嘧啶抑制细胞质核糖体 RNA 合成后，激动素对延缓叶片衰老的作用和脱落酸对加速叶片衰老的作用并没有改变。用 5-氟尿嘧啶抑制细胞质核糖体 RNA 合成后，激动素刺激并脱落酸抑制放射性掺入多分散 RNA。这些结果与激动素和脱落酸在衰老叶片组织中的可能作用模式有关。
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