Pohlmann J
Institut für Allgemeine Botanik der Universität Hamburg, Hamburg, Deutschland.
Planta. 1971 Mar;100(1):31-46. doi: 10.1007/BF00386885.
A synergism of colchicine and different cucurbitacins has been shown, to be a cause in the formation of ball-metaphases in Bryonia species. The bitter principles cucurbitacin L and I, dihydrocucurbitacin B, and tetrahydrocucurbitacin I, which were examined more closely with respect to their karyological effects, were shown to give rise to C-metaphases in Hordeum and Vicia at concentrations of about 0.016 per cent. On the one hand the number of mitotic stages is reduced, and on the other hand there is an increase of metaphase stages. Higher concentrations lead to pycnotic degenerations of nuclei and are toxic. When colchicine is applied together with cucurbitacins, ball-metaphases result. This could be demonstrated in plants lacking colchicine and cucurbitacins (Hordeum) when both substances were applied at the same time. Ball-metaphases also appear in plants containing cucurbitacins (Bryonia alba, B. dioica, Citrullus colocythis, Iberis amara) when they are treated with colchicine.
秋水仙碱与不同的葫芦素之间存在协同作用，这已被证明是泻根属植物中形成球状中期的一个原因。对葫芦素L和I、二氢葫芦素B以及四氢葫芦素I的核学效应进行了更深入研究，结果表明，在浓度约为0.016%时，它们会在大麦和蚕豆中产生C中期。一方面，有丝分裂阶段的数量减少，另一方面，中期阶段有所增加。更高的浓度会导致细胞核固缩变性，具有毒性。当秋水仙碱与葫芦素一起使用时，会产生球状中期。当同时施用这两种物质时，在缺乏秋水仙碱和葫芦素的植物（大麦）中可以证明这一点。当用秋水仙碱处理时，含有葫芦素的植物（白泻根、二叶泻根、刺角瓜、屈曲花）也会出现球状中期。
