Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Mol Genet Genomics. 2014 Jun;289(3):361-72. doi: 10.1007/s00438-014-0815-7. Epub 2014 Feb 1.
The KIN genes are crucial members of the cold-regulated gene family. They play exclusive roles during the developmental processes of many organs and respond to various abiotic stresses in plants. However, little is known about the regulation of KIN gene expression in cytoplasmic male sterility (CMS) cabbages (Brassica oleracea L. var. capitata L.). We carried out a genome-wide analysis to identify the KIN genes in the CMS cabbage. Two non-redundant KIN genes, named BoKIN1 (Bol021262) and BoKIN2 (Bol030498), were identified. Reverse transcriptase PCR detected alternative splicing (AS) products of BoKIN1 (four AS products) and BoKIN2 (three AS products). In addition, alternative polyadenylation (APA) was observed for BoKIN1 and BoKIN2 in the CMS cabbage, resulting in variable 3'UTRs in their transcripts. Furthermore, the transcription levels of BoKIN1-0 and BoKIN2-0, the introns of which were spliced completely, were analyzed in various organs and young leaves treated by abiotic stresses. Our data indicated that BoKIN1-0 is highly expressed in various organs, whereas BoKIN2-0 is expressed exclusively in the stamen. Our study also suggested that BoKIN1-0 was upregulated significantly in young leaves of plants exposed to abscisic acid treatment, and cold and heat stress. BoKIN1 and BoKIN2 had differential AS and APA patterns in pre-mRNA processing, and showed differences in their expression patterns and transcript levels. BoKIN1 participates widely in organ development and responds to diverse abiotic stresses, whereas BoKIN2 plays a main role in stamen development in the CMS cabbage.
KIN 基因是冷调控基因家族的重要成员。它们在许多器官的发育过程中发挥独特的作用，并对植物中的各种非生物胁迫做出响应。然而，关于细胞质雄性不育（CMS）白菜中 KIN 基因表达的调控知之甚少。我们进行了全基因组分析，以鉴定 CMS 白菜中的 KIN 基因。鉴定出两个非冗余的 KIN 基因，命名为 BoKIN1（Bol021262）和 BoKIN2（Bol030498）。逆转录 PCR 检测到 BoKIN1（四种 AS 产物）和 BoKIN2（三种 AS 产物）的可变剪接（AS）产物。此外，CMS 白菜中观察到 BoKIN1 和 BoKIN2 的交替多聚腺苷酸化（APA），导致其转录物的 3'UTR 可变。此外，分析了完全拼接内含子的 BoKIN1-0 和 BoKIN2-0 的转录水平，以及各种器官和受到非生物胁迫处理的幼叶。我们的数据表明，BoKIN1-0 在各种器官中高度表达，而 BoKIN2-0 仅在雄蕊中表达。我们的研究还表明，ABA 处理、冷和热胁迫下，暴露于胁迫的植物幼叶中 BoKIN1-0 显著上调。BoKIN1 和 BoKIN2 在 pre-mRNA 加工中具有不同的 AS 和 APA 模式，并且在表达模式和转录水平上表现出差异。BoKIN1 广泛参与器官发育，并响应多种非生物胁迫，而 BoKIN2 在 CMS 白菜的雄蕊发育中起主要作用。
