Bishop A H, Rachwal P A
Detection Department, Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, SP4 0JQ, UK,
Curr Microbiol. 2014 Jun;68(6):693-701. doi: 10.1007/s00284-014-0526-7. Epub 2014 Feb 2.
Transposon-directed insertion site sequencing was used to identify genes required by Burkholderia thailandensis to survive in plant/soil microcosms. A total of 1,153 genetic loci fulfilled the criteria as being likely to encode survival characteristics. Of these, 203 (17.6 %) were associated with uptake and transport systems; 463 loci (40.1 %) coded for enzymatic properties, 99 of these (21.4 %) had reduction/oxidation functions; 117 (10.1 %) were gene regulation or sensory loci; 61 (5.3 %) encoded structural proteins found in the cell envelope or with enzymatic activities related to it, distinct from these, 46 (4.0 %) were involved in chemotaxis and flagellum, or pilus synthesis; 39 (3.4 %) were transposase enzymes or were bacteriophage-derived; and 30 (2.6 %) were involved in the production of antibiotics or siderophores. Two hundred and twenty genes (19.1 %) encoded hypothetical proteins or those of unknown function. Given the importance of motility and pilus formation in microcosm persistence the nature of the colonization of the rhizosphere was examined by confocal microscopy. Wild type B. thailandensis expressing red fluorescent protein was inoculated into microcosms. Even though the roots had been washed, the bacteria were still present but they were motile with no attachment having taken place, perhaps being retained in a biofilm.
转座子导向的插入位点测序被用于鉴定泰国伯克霍尔德菌在植物/土壤微观环境中生存所需的基因。共有1153个基因座符合可能编码生存特性的标准。其中，203个（17.6%）与摄取和运输系统相关；463个基因座（40.1%）编码酶的特性，其中99个（21.4%）具有还原/氧化功能；117个（10.1%）是基因调控或传感基因座；61个（5.3%）编码在细胞膜中发现的结构蛋白或与之相关的酶活性，除此之外，46个（4.0%）参与趋化作用、鞭毛或菌毛合成；39个（3.4%）是转座酶或来源于噬菌体；30个（2.6%）参与抗生素或铁载体的产生。220个基因（19.1%）编码假设的蛋白质或功能未知的蛋白质。鉴于运动性和菌毛形成在微观环境持久性中的重要性，通过共聚焦显微镜检查了根际定殖的性质。将表达红色荧光蛋白的野生型泰国伯克霍尔德菌接种到微观环境中。尽管根已被冲洗，但细菌仍然存在，但它们具有运动性，没有发生附着，可能保留在生物膜中。
