Bartelmess P, Bengtsson F, Nobin A, Jeppsson B, Herlin P, Bugge M
Dept. of Surgery, University of Lund, Sweden.
Res Exp Med (Berl). 1987;187(5):353-8. doi: 10.1007/BF01855661.
In rats with a portacaval shunt (PCS), the effect on the serotonin metabolism in the brain after oral administration of blood, a mixed amino acid solution (Vamin 14; KabiVitrum, Sweden) or a 10% glucose solution was studied. One week after PCS, the animals were fed with a gastric tube for 8 h and thereafter tested for behavioral abnormalities before decapitation at 12 h. The concentration of 5-hydroxytryptophan (5-HTP), serotonin (5-HT), and 5-hydroxyindoleacetic acid (5-HIAA) were analyzed chromatographically (HPLC technique with electrochemical detection) in different regions of the brain. Estimation of synthetic rates of 5-hydroxyindoles was facilitated by aromatic aminoacid decarboxylase inhibition (m-hydroxybenzyl-hydrazine; NSD 1015). The brain concentrations of 5-HTP, 5-HT, and 5-HIAA were increased in all shunted rats as compared with sham-operated animals. Whether animals received blood, glucose, or aminoacid solution made no differences in the brain concentrations of 5-HTP and 5-HT. Concentrations of 5-HIAA were lower in those animals receiving blood as compared with the other shunted groups. No reproducible differences in the behavior of the animals were observed. These results suggest that massive blood administration 1 week after PCS in rats has no influence on the rate of brain indole synthesis. While alterations in serotonin metabolism may play a role in some forms of encephalopathy, this study implies that the behavioral and neurologic disorders which follow gastrointestinal tract hemorrhage in patients with liver failure may have other etiologies.
在患有门腔分流术（PCS）的大鼠中，研究了口服血液、混合氨基酸溶液（Vamin 14；瑞典卡比维特鲁姆公司）或10%葡萄糖溶液后对大脑中血清素代谢的影响。PCS术后一周，通过胃管对动物喂食8小时，然后在12小时断头前测试行为异常情况。采用高效液相色谱电化学检测技术，对大脑不同区域的5-羟色氨酸（5-HTP）、血清素（5-HT）和5-羟吲哚乙酸（5-HIAA）浓度进行色谱分析。通过抑制芳香族氨基酸脱羧酶（间羟基苄基肼；NSD 1015）来促进5-羟吲哚合成速率的估算。与假手术动物相比，所有分流大鼠大脑中的5-HTP、5-HT和5-HIAA浓度均升高。动物接受血液、葡萄糖或氨基酸溶液对大脑中5-HTP和5-HT的浓度没有影响。与其他分流组相比，接受血液的动物5-HIAA浓度较低。未观察到动物行为有可重复的差异。这些结果表明，大鼠PCS术后1周大量输血对大脑吲哚合成速率没有影响。虽然血清素代谢改变可能在某些形式的脑病中起作用，但本研究表明，肝功能衰竭患者胃肠道出血后出现的行为和神经紊乱可能有其他病因。
