Bhatia P, Kochupillai V, Mathew S, Mehra N K, Nanu A, Jayasuryan N, Sharma S, Francis S, Menon P S
Medical Oncology Department, Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi.
Thymus. 1987;10(1-2):125-30. doi: 10.1007/978-94-009-3365-1_14.
Studies to find engraftment following fetal liver infusion (FLI) in aplastic anaemia (AA) and acute myeloid leukaemia (AML) were carried out in 24 patients (17 AA and 7 AML patients) out of the 56 who received FLI. HLA studies done in 13 patients (3 AA and 5 AML), repeatedly after FLI, showed no significant change in HLA antigen pattern before and after FLI. Red cell antigen studies were done in five (1 AA and 4 AML) patients, 3 weeks to 7 months after FLI. One patient with AML who was Rh negative prior to reinduction chemotherapy became Rh positive two months after FLI; six months later he was Rh negative again. In the remaining patients there was no change in red cell antigen pattern after FLI. Radio-immuno-assay to detect alpha-fetoprotein levels, carried out in 10 (8 AA and 2 AML) patients repeatedly after FLI, demonstrated no increase. In 13 patients (8 AA and 5 AML) in whom there was a sex difference between donor and recipient, bone marrow cultures for sex chromosomes revealed mixture of XX and XY cells in 3 male patients with aplastic anaemia. One male patient with AML demonstrated complete engraftment after induction chemotherapy and FLI: all the mitoses studied were of XX pattern. Engraftment was however temporary as repeated studies revealed reversion to XY pattern. The present work suggests that infusion of fetal liver cells may sometimes induce temporary chimerism or engraftment in an adult host; in the majority of cases, however, engraftment could not be established.
在接受胎儿肝脏输注（FLI）的56例患者中，对24例（17例再生障碍性贫血患者和7例急性髓系白血病患者）进行了关于再生障碍性贫血（AA）和急性髓系白血病（AML）中FLI后植入情况的研究。对13例患者（3例AA患者和5例AML患者）在FLI后多次进行HLA研究，结果显示FLI前后HLA抗原模式无显著变化。对5例患者（1例AA患者和4例AML患者）在FLI后3周至7个月进行红细胞抗原研究。1例AML患者在诱导化疗前为Rh阴性，FLI后2个月变为Rh阳性；6个月后又变为Rh阴性。其余患者FLI后红细胞抗原模式无变化。对10例患者（8例AA患者和2例AML患者）在FLI后多次进行放射免疫测定以检测甲胎蛋白水平，结果显示无升高。在13例供体和受体存在性别差异的患者（8例AA患者和5例AML患者）中，对性染色体进行骨髓培养，发现3例再生障碍性贫血男性患者的细胞中XX和XY细胞混合存在。1例AML男性患者在诱导化疗和FLI后显示完全植入：所有研究的有丝分裂均为XX模式。然而，植入是暂时的，因为重复研究显示又恢复为XY模式。目前的研究表明，输注胎儿肝细胞有时可能在成年宿主中诱导暂时的嵌合或植入；然而，在大多数情况下，无法建立植入。
