de Lima Marcelo Lopes, Bertti Rodolfo, Moro Juliano César, Coltro Neto Fábio, Miyaoka Ricardo, Fregonesi Adriano, de Lima Mariana da Cunha Lopes, Ramos Celso Darío
Division of Urology, Department of Surgery, Campinas State University, Avenida Vital Brasil 251, Caixa Postal 6142, Cidade Universitária Zeferino Vaz, 13083-888 Campinas, SP, Brazil.
Nuclear Medicine Division, Department of Radiology, Campinas State University, Avenida Vital Brasil 251, Caixa Postal 6142, Cidade Universitária Zeferino Vaz, 13083-888 Campinas, SP, Brazil.
Adv Urol. 2013;2013:929620. doi: 10.1155/2013/929620. Epub 2013 Dec 29.
Objective. To create a ureteral obstruction experimental model that can be proved through (99m)Tc-DTPA renal scintigraphy and histopathological studies, without causing total renal function loss. Materials and Methods. Ten New Zealand white rabbits were submitted to a surgical experiment to create a model of unilateral obstruction to urinary flow. Surgery procedure provided unilateral ureteral obstruction (left kidney) to urinary flow and posteriorly was evaluated by (99m)Tc-DTPA renal scintigraphy and histopathological study. (99m)Tc-DTPA renal study was performed to detect and quantify signs of obstruction and to evaluate renal function. Statistical analysis was performed through the Student t-test with a significance level of P<0.05. Results. Nine of the ten rabbits presented left renal unit obstruction and one nonobstructive on the (99m)Tc-DTPA and histopathological studies. All the right renal units, which were not submitted to surgical procedure, were nonobstructed by the studies. There was a general agreement between scintigraphy and histopathological results in both groups. Conclusion. The experimental model promoted the creation of ureteral obstruction in rabbits, confirmed by nuclear medicine scintigraphy and histopathology, and could be used in further studies to better understand urinary obstruction.
目的。创建一种输尿管梗阻实验模型，该模型可通过（99m）Tc-DTPA肾闪烁显像和组织病理学研究得到证实，且不会导致肾功能完全丧失。材料与方法。对10只新西兰白兔进行手术实验以创建单侧尿路梗阻模型。手术操作造成单侧（左肾）输尿管尿路梗阻，术后通过（99m）Tc-DTPA肾闪烁显像和组织病理学研究进行评估。进行（99m）Tc-DTPA肾研究以检测和量化梗阻迹象并评估肾功能。采用Student t检验进行统计分析，显著性水平为P<0.05。结果。在（99m）Tc-DTPA和组织病理学研究中，10只兔子中有9只出现左肾单位梗阻，1只无梗阻。所有未接受手术操作的右肾单位在研究中均无梗阻。两组的闪烁显像和组织病理学结果总体一致。结论。该实验模型成功在兔子中造成了输尿管梗阻，经核医学闪烁显像和组织病理学证实，可用于进一步研究以更好地理解尿路梗阻。
