Department of Biological Science and Technology, National Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan.
Department of Biological Science and Technology, National Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan ; Department of Agricultural Product Technology, Brawijaya University, Jalan, Veteran Malang 65145, Indonesia.
Evid Based Complement Alternat Med. 2013;2013:857213. doi: 10.1155/2013/857213. Epub 2013 Dec 10.
Fatsia polycarpa, a plant endemic to Taiwan, is an herbal medicine known for treating several inflammation-related diseases, but its biological function needs scientific support. Thus, the anti-inflammatory effects and mechanisms of the methanolic crude extract (MCE) of F. polycarpa and its feature constituents, that is, brassicasterol (a phytosterol), triterpenoids 3 α -hydroxyolean-11,13(18)-dien-28-oic acid (HODA), 3 α -hydroxyolean-11-en-28,13 β -olide (HOEO), fatsicarpain D, and fatsicarpain F, were investigated. MCE and HOEO, but not brassicasterol, dose-dependently inhibited lipopolysaccharide- (LPS-)induced expression of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 macrophage line, whereas HODA, fatsicarpain D and fatsicarpain F were toxic to RAW cells. Additionally, MCE and HOEO suppressed LPS-induced production of nitric oxide, prostaglandin E2, and interleukin-1 β and interfered with LPS-promoted activation of the inhibitor kappa B kinase (IKK)/nuclear factor- κ B (NF- κ B) pathway, and that of the mitogen-activated protein kinases (MAPKs) extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. In animal tests, MCE and HOEO effectively ameliorated 12-O-tetradecanoylphorobol-13 acetate- (TPA-)induced ear edema of mice. Thus, MCE of F. polycarpa exhibited an obvious anti-inflammatory activity in vivo and in vitro that likely involved the inhibition of the IKK/NF- κ B pathway and the MAPKs, which may be attributed by triterpenoids such as HOEO.
海桐花，一种台湾特有的植物，是一种草药，用于治疗多种炎症相关疾病，但它的生物学功能需要科学支持。因此，研究了海桐花甲醇粗提取物（MCE）及其特征成分，即菜籽固醇（一种植物固醇）、三萜 3α-羟基齐墩果酸 11,13(18)-二烯-28-酸（HODA）、3α-羟基齐墩 11-烯-28,13β-内酯（HOEO）、海桐花脂素 D 和海桐花脂素 F 的抗炎作用及其机制。MCE 和 HOEO 而非菜籽固醇可剂量依赖性地抑制脂多糖（LPS）诱导的 RAW264.7 巨噬细胞系中诱导型一氧化氮合酶和环氧化酶-2 的表达，而 HODA、海桐花脂素 D 和海桐花脂素 F 对 RAW 细胞有毒性。此外，MCE 和 HOEO 抑制 LPS 诱导的一氧化氮、前列腺素 E2 和白细胞介素-1β的产生，并干扰 LPS 促进的抑制剂κB 激酶（IKK）/核因子-κB（NF-κB）途径以及丝裂原激活蛋白激酶（MAPKs）细胞外信号调节激酶（ERK）、c-Jun N 末端激酶（JNK）和 p38 的激活。在动物试验中，MCE 和 HOEO 有效改善了 12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的小鼠耳肿胀。因此，海桐花 MCE 在体内和体外均表现出明显的抗炎活性，这可能涉及 IKK/NF-κB 途径和 MAPKs 的抑制，这可能归因于三萜类化合物如 HOEO。
