Yang Tsung-Lin, Lin Lin, Lou Pei-Jen, Chang Ta-Chau, Young Tai-Horng
Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan ; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan.
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.
PLoS One. 2014 Jan 28;9(1):e86143. doi: 10.1371/journal.pone.0086143. eCollection 2014.
Cancer can be easily treated when found early. A probe capable of detecting cell transformation may increase the success rate of early diagnosis of cancer. In this report we have tested the ability of a fluorescent, quadruplex DNA binding probe, 3,6-bis(1-methyl-4- vinylpyridinium) carbazole diiodide (BMVC), to detect cell transformation in vitro. BMVC was applied to living cells in several different models of cell transformation, and the fluorescence signals of BMVC were measured. The degrees of cell transformation in these models were characterized by alterations in cellular morphological phenotype and subcellular organization. When BMVC probes were applied, the number of BMVC-positive cells increased in accordance with the degree of transformation. BMVC was capable of significantly detecting formation of foci, increased cellular motility, cell proliferation, cell apoptosis, anchorage-independent growth, and increased invasiveness of transformed cells. These results demonstrate the ability of BMVC probes to detect cell transformation and indicate that BMVC is of promise for use as a probe in early cancer detection.
癌症若早期发现，很容易治疗。一种能够检测细胞转化的探针可能会提高癌症早期诊断的成功率。在本报告中，我们测试了一种荧光四链体DNA结合探针3,6-双(1-甲基-4-乙烯基吡啶鎓)咔唑二碘化物(BMVC)在体外检测细胞转化的能力。BMVC被应用于几种不同的细胞转化模型中的活细胞，并测量了BMVC的荧光信号。这些模型中的细胞转化程度通过细胞形态表型和亚细胞组织的改变来表征。当应用BMVC探针时，BMVC阳性细胞的数量随着转化程度的增加而增加。BMVC能够显著检测到病灶的形成、细胞运动性增加、细胞增殖、细胞凋亡、不依赖贴壁生长以及转化细胞侵袭性增加。这些结果证明了BMVC探针检测细胞转化的能力，并表明BMVC有望用作早期癌症检测的探针。
