Nghiem L N, Bánfalvi G, Csuka I, Oláh I, Antoni F
Institute of Biochemistry Department I, Semmelweis University Medical School, Budapest, Hungary.
Acta Biochim Biophys Hung. 1987;22(1):75-83.
The in vitro effect of antiparasitic agents, emetine and chloroquine on the DNA, RNA and protein synthesis was studied in isolated murine thymocytes. Cytotoxic effect was observed at 10(-4) and 10(-3) M emetine concentrations causing 50% and 70% cell death, respectively. The toxic effect of emetine could be prevented when emetine was removed within 10 min of treatment. Inhibitory concentrations for DNA, RNA and protein biosynthesis were 10(-5), 5 x 10(-5) and 10(-8) M, respectively. The number of living cells decreased by 30 and 50% at 10(-4) and 10(-3) M chloroquine concentrations, respectively. Gradually decreasing rate of DNA synthesis was measured at increasing concentration of chloroquine between 10(-8) and 10(-3) while RNA and protein synthesis were effected at 5 x 10(-5) M concentration. These results indicate that protein biosynthesis is primarily affected by in vitro emetine and chloroquine treatment of murine thymocytes.
在分离的小鼠胸腺细胞中研究了抗寄生虫药吐根碱和氯喹对DNA、RNA和蛋白质合成的体外作用。在吐根碱浓度为10⁻⁴和10⁻³M时观察到细胞毒性作用，分别导致50%和70%的细胞死亡。如果在处理后10分钟内去除吐根碱，其毒性作用可以避免。DNA、RNA和蛋白质生物合成的抑制浓度分别为10⁻⁵、5×10⁻⁵和10⁻⁸M。氯喹浓度为10⁻⁴和10⁻³M时，活细胞数量分别减少30%和50%。在氯喹浓度从10⁻⁸增加到10⁻³M的过程中，DNA合成速率逐渐降低，而RNA和蛋白质合成在5×10⁻⁵M浓度时受到影响。这些结果表明，蛋白质生物合成主要受体外吐根碱和氯喹处理小鼠胸腺细胞的影响。
