吐根碱和氯喹对小鼠胸腺细胞大分子生物合成的体外作用。

In vitro effect of emetine and chloroquine on the macromolecular biosynthesis of murine thymus cells.

作者信息

Nghiem L N, Bánfalvi G, Csuka I, Oláh I, Antoni F

机构信息

Institute of Biochemistry Department I, Semmelweis University Medical School, Budapest, Hungary.

出版信息

Acta Biochim Biophys Hung. 1987;22(1):75-83.

PMID:2448981

Abstract

The in vitro effect of antiparasitic agents, emetine and chloroquine on the DNA, RNA and protein synthesis was studied in isolated murine thymocytes. Cytotoxic effect was observed at 10(-4) and 10(-3) M emetine concentrations causing 50% and 70% cell death, respectively. The toxic effect of emetine could be prevented when emetine was removed within 10 min of treatment. Inhibitory concentrations for DNA, RNA and protein biosynthesis were 10(-5), 5 x 10(-5) and 10(-8) M, respectively. The number of living cells decreased by 30 and 50% at 10(-4) and 10(-3) M chloroquine concentrations, respectively. Gradually decreasing rate of DNA synthesis was measured at increasing concentration of chloroquine between 10(-8) and 10(-3) while RNA and protein synthesis were effected at 5 x 10(-5) M concentration. These results indicate that protein biosynthesis is primarily affected by in vitro emetine and chloroquine treatment of murine thymocytes.

摘要

在分离的小鼠胸腺细胞中研究了抗寄生虫药吐根碱和氯喹对DNA、RNA和蛋白质合成的体外作用。在吐根碱浓度为10⁻⁴和10⁻³M时观察到细胞毒性作用，分别导致50%和70%的细胞死亡。如果在处理后10分钟内去除吐根碱，其毒性作用可以避免。DNA、RNA和蛋白质生物合成的抑制浓度分别为10⁻⁵、5×10⁻⁵和10⁻⁸M。氯喹浓度为10⁻⁴和10⁻³M时，活细胞数量分别减少30%和50%。在氯喹浓度从10⁻⁸增加到10⁻³M的过程中，DNA合成速率逐渐降低，而RNA和蛋白质合成在5×10⁻⁵M浓度时受到影响。这些结果表明，蛋白质生物合成主要受体外吐根碱和氯喹处理小鼠胸腺细胞的影响。