Broekaert D, Van Oostveldt P, Coucke P, Reyniers P, Kluyskens P, Gillis E
Laboratory of Physiological Chemistry, Faculty of Medicine, State University of Ghent, Belgium.
Acta Otolaryngol. 1988 Jan-Feb;105(1-2):90-9. doi: 10.3109/00016488809119450.
Quantitative DNA cytophotometric techniques were applied to judge the alteration (differentiation) and ultimate fate of nuclei during keratinization in human middle ear cholesteatoma. Compared with a healthy epidermis, a tendency towards postponed nuclear degradation was noticed. Two patterns governing the loss of DNA are recognized. In one group, the mean nuclear DNA content declines continuously, starting in the nearest suprabasal layers and continuing throughout the prickle and granular cell stages, where the ultimate degeneration of nuclei takes place. This pathway corresponds to that observed in epidermis, but evolves more slowly. In another group of samples, the onset of the DNA decline is delayed to the upper prickle cells, exceptionally to more terminal stages of keratinization. During matrix keratinization, a profound nuclear remodelling takes place, similar to that in epidermal tissues, as far as eu- and heterchromatin DNA and area data are concerned. However, euchromatinization of nuclei in matrix prickle cells is more pronounced than in epidermal tissues. The topography of residual heterochromatic clumps does not reflect a persistent margination as in epidermal nuclei, but is the result of more individualized rearrangements. The changes in karyotype are less elaborate when the complete decline of the nuclear DNA content only occurs during terminal keratinization.
应用定量DNA细胞光度测量技术来判断人中耳胆脂瘤角化过程中细胞核的变化（分化）及最终命运。与健康表皮相比，发现有核降解延迟的趋势。识别出两种控制DNA丢失的模式。在一组中，平均核DNA含量持续下降，始于最接近基底层上方的细胞层，并在棘层和颗粒细胞阶段持续，细胞核最终在此处发生退变。此途径与在表皮中观察到的一致，但进展更为缓慢。在另一组样本中，DNA下降的起始延迟至上棘层细胞，极少数情况下延迟至角化的更终末期。在基质角化过程中，就常染色质和异染色质DNA及面积数据而言，发生了与表皮组织中类似的深刻核重塑。然而，基质棘层细胞核的常染色质化比表皮组织中更为明显。残余异染色质团块的拓扑结构并不像表皮细胞核那样反映出持续的边缘化，而是更个体化重排的结果。当核DNA含量仅在终末角化期间完全下降时，核型变化则不那么复杂。
