Broekaert D, Pattin C, Coucke P, De Bersaques J, Marquet J
Laboratory of Physiological Chemistry, Faculty of Medicine, State University of Ghent, Belgium.
Eur Arch Otorhinolaryngol. 1990;247(5):312-7. doi: 10.1007/BF00176544.
A histochemical study was performed to determine the involvement of epidermal transglutaminase (ETgase) in the keratinization of middle ear cholesteatomatous lesions, and to compare it with its role in the middle ear mucosa and epidermis. In a first assay, we localized the (E)Tgase activity in situ. A second immunohistochemical assay revealed the distribution of the particulate form of ETgase, which is involved in cross-linked envelope formation. A remarkable difference between strongly keratinized epidermal tissues and the cholesteatoma matrix is the frequent observation in the latter of the remnants of (E)Tgase activity in cytosol, even in advanced stages of differentiation. As a consequence, the cell-membrane-associated ETgase activity, and thus the extent of cross-linking within the envelope, is at a lower level than expected. This aspect is reminiscent of the keratinization phenomenon manifested by thin epidermal tissues. In addition, our findings are the first to show that ETgase is a substantial marker of middle ear mucosa.
进行了一项组织化学研究，以确定表皮转谷氨酰胺酶（ETgase）在中耳胆脂瘤病变角质化过程中的作用，并将其与在中耳黏膜和表皮中的作用进行比较。在第一项分析中，我们对（E）Tgase活性进行了原位定位。第二项免疫组织化学分析揭示了参与交联包膜形成的颗粒形式ETgase的分布。高度角质化的表皮组织与胆脂瘤基质之间的一个显著差异是，即使在分化的晚期阶段，在胆脂瘤基质的细胞质中也经常观察到（E）Tgase活性的残余。因此，与细胞膜相关的ETgase活性以及包膜内的交联程度低于预期。这一现象让人联想到薄表皮组织所表现出的角质化现象。此外，我们的研究结果首次表明ETgase是中耳黏膜的一个重要标志物。
