Li Dong-Bin, Gao Han-Hui, Si Jin-Ping, Zhu Yu-Qiu
Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
Zhongguo Zhong Yao Za Zhi. 2013 Oct;38(20):3446-52.
To investigate HSP70 gene expression from Dendrobium officinale under low temperature stress, which will provide the molecular biological foundation for breeding the low temperature resistant strain.
HSP70 gene full length cDNA was cloned by rapid amplification of cDNA ends (RACE) on the basis of HSP70 gene fragment sequences, and the structure and function of HSP70 gene were deduced. The expression of HSP70 under low temperature stress was detected by RT-PCR.
The full length of HSP70 gene cDNA was 2 296 bp containing a 1 944 bp open reading frame (ORF) that encoded a protein of 647 amino acids. Its amino acids sequence had typical HSP70 characteristics and high homology with other plant's HSP70. Cold stress expression analysis showed that expression of the HSP70 gene could be induced by low temperature.
The HSP70 gene of D. officinale was successfully cloned and reported for the first time which proved that the expression could be induced by low temperature. The cloning of HSP70 gene provides a stable foundation for further study of D. officinale cultivation and the breeding of the cold resistance strains.
研究低温胁迫下铁皮石斛HSP70基因的表达情况，为培育耐低温品系提供分子生物学依据。
根据HSP70基因片段序列，采用cDNA末端快速扩增技术（RACE）克隆HSP70基因全长cDNA，并推导其结构与功能。通过RT-PCR检测低温胁迫下HSP70的表达情况。
HSP70基因cDNA全长2 296 bp，包含一个1 944 bp的开放阅读框（ORF），编码一个由647个氨基酸组成的蛋白质。其氨基酸序列具有典型的HSP70特征，与其他植物的HSP70具有高度同源性。低温胁迫表达分析表明，HSP70基因的表达可被低温诱导。
首次成功克隆并报道了铁皮石斛的HSP70基因，证明其表达可被低温诱导。HSP70基因的克隆为进一步研究铁皮石斛栽培及抗寒品系选育奠定了坚实基础。
