Li Dong-Bin, Gao Yan-Hui, Si Jin-Ping
Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Provinical Strategic Allance for Technical Innovation in Industry of Dendrobium officinale Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
Zhongguo Zhong Yao Za Zhi. 2013 Feb;38(4):511-5.
In order to study the molecule mechanism of the differential expression in Dendrobium officinale under low temperature, the high cold resistance germplasms were used for constructing the RNA pools. SCoT markers were used to analyze the different cDNA pools transcribed from the RNA pools. 11 transcripts derived fragments from 500 cDNA amplified bands were amplified by 64 primers, and were sorted out, cloned, sequenced and analyzed. The results showed that cDNA pools with SCoT markers could be used for differential display in D. officinale under low temperature stress. Sequence analysis indicated that the transcripts derived fragments were significantly homologous in nucleotide sequence with membrane-associated proteins, osmotic regulation protein, CBF transcriptional factor, resistance protein. One left gene segments functions were still unknown, which may be related to the cold resistant gene expression in D. officinale.
为研究低温下铁皮石斛差异表达的分子机制，以高抗寒种质构建RNA池。利用SCoT标记分析从RNA池转录而来的不同cDNA池。用64条引物从500条cDNA扩增条带中扩增出11个转录本衍生片段，进行筛选、克隆、测序和分析。结果表明，带有SCoT标记的cDNA池可用于低温胁迫下铁皮石斛的差异显示。序列分析表明，转录本衍生片段在核苷酸序列上与膜相关蛋白、渗透调节蛋白、CBF转录因子、抗性蛋白具有显著同源性。有一个基因片段功能尚不清楚，可能与铁皮石斛抗寒基因表达有关。
