Kass L
Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio.
Am J Clin Pathol. 1988 Feb;89(2):195-202. doi: 10.1093/ajcp/89.2.195.
This report describes the selective staining of lysosomes in cells of neutrophilic granulocytic origin by the azo textile dye basic blue 93. Used as a single agent aqueous stain after fixation, the dye stained lysosomes black. Stained in this way, lysosomes were sharply delimited and easily visualized. Large numbers of black-stained lysosomes occurred in immature granulocytes like promyelocytes and myelocytes. In more mature granulocytes like metamyelocytes, bands, and neutrophils, substantially fewer numbers of lysosomes were detected. In leukemic myeloblasts and promyelocytes stained with basic blue 93, variable numbers of black-colored lysosomes could be detected and usually paralleled the reactions for myeloperoxidase and Sudan black B in the same cells. As an alternative to the traditional stains for myeloperoxidase, basic blue 93 has several advantages, including simplicity of use, ability to stain aged specimens, and stability of the reaction product.
本报告描述了偶氮纺织染料碱性蓝93对嗜中性粒细胞来源的细胞中的溶酶体进行选择性染色的情况。该染料在固定后用作单一的水性染色剂，可将溶酶体染成黑色。以这种方式染色后，溶酶体界限清晰，易于观察。大量黑色染色的溶酶体出现在未成熟粒细胞中，如早幼粒细胞和中幼粒细胞。在更成熟的粒细胞中，如晚幼粒细胞、带状核粒细胞和中性粒细胞中，检测到的溶酶体数量明显较少。在用碱性蓝93染色的白血病原始粒细胞和早幼粒细胞中，可以检测到数量不等的黑色溶酶体，且通常与同一细胞中髓过氧化物酶和苏丹黑B的反应情况相似。作为髓过氧化物酶传统染色方法的替代方法，碱性蓝93具有几个优点，包括使用简便、能够对陈旧标本进行染色以及反应产物的稳定性。
