Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea 4928.
J Food Prot. 2014 Feb;77(2):339-44. doi: 10.4315/0362-028X.JFP-13-133.
The objective of this study was to evaluate the effect of different concentrations of reconstituted apple and orange juice on reduction of Alicyclobacillus acidoterrestris spores by gamma irradiation. Spores of A. acidoterrestris were inoculated into three concentrations of apple (18, 36, and 72 °Brix) and orange (11, 33, and 66 °Brix) juice and subjected to five radiation doses (1, 3, 5, 7, and 10 kGy). No significant reductions (P > 0.05) in spores were observed after the 1-kGy treatment for all apple and orange concentrations. Spores in 18, 36, and 72 °Brix apple juice concentrates subjected to 10 kGy were reduced to 4.34, 3.9, and 3.84 log CFU/ml, respectively. Similar results were observed for orange juice. When 10 kGy was applied to 11 °Brix orange juice, populations of spores were reduced by 5 log CFU/ml. The reduction of spores in 33 and 66 °Brix orange juice concentrates exposed to 10-kGy gamma irradiation was 4.54 and 3.85 log CFU/ml, respectively. Juice concentration did not affect (P > 0.05) the number of surviving A. acidoterrestris spores from the same kGy treatment. Gamma irradiation treatment did not change the pH or water activity of the juice (P > 0.05).
本研究旨在评估不同浓度调配苹果汁和橙汁对γ射线辐照降低耐热性环状芽孢杆菌孢子的影响。将耐热性环状芽孢杆菌孢子接种到三种浓度的苹果汁（18°Brix、36°Brix 和 72°Brix）和橙汁（11°Brix、33°Brix 和 66°Brix）中，并接受五个辐射剂量（1、3、5、7 和 10 kGy）。在所有苹果和橙汁浓度的 1 kGy 处理后，观察到孢子没有明显减少（P＞0.05）。在 10 kGy 处理下，18°Brix、36°Brix 和 72°Brix 苹果汁浓缩液中的孢子分别减少至 4.34、3.9 和 3.84 log CFU/ml。橙汁也观察到类似的结果。当 10 kGy 应用于 11°Brix 橙汁时，孢子数量减少了 5 log CFU/ml。在 33°Brix 和 66°Brix 橙汁浓缩液中，暴露于 10 kGy γ射线辐照的孢子减少了 4.54 和 3.85 log CFU/ml。辐照处理对相同辐照剂量下存活的耐热性环状芽孢杆菌孢子数量没有影响（P＞0.05）。γ射线辐照处理未改变果汁的 pH 值或水活度（P＞0.05）。
