Egger D, Bienz K
Institute for Microbiology, University of Basel, Switzerland.
Anal Biochem. 1987 Nov 1;166(2):413-7. doi: 10.1016/0003-2697(87)90592-6.
Proteins blotted onto nitrocellulose can be stained with colloidal gold. They retain their immunoreactivity so that the stained blot can be probed with appropriate antibodies. The resulting antigen-antibody reaction is made visible by a peroxidase-coupled antispecies antibody. The blue peroxidase reaction product contrasts well with the red-stained protein pattern and allows an easy documentation by black-and-white photography.
