Egger D, Bienz K
Institute for Medical Microbiology, University of Basel, Switzerland.
Mol Biotechnol. 1994 Jun;1(3):289-305. doi: 10.1007/BF02921696.
The different steps involved in protein (Western) blotting and subsequent analysis of the proteins are reviewed. Electrophoretic separation of proteins, procedures of transfer to membranes, immunological and nonimmunological protein detection systems, and characterization of protein-nucleic acid and protein-protein interactions are described. Emphasis is on the sensitivity of the methods described and on possible variations that allow the individual steps of Western blotting to be adapted to specific questions.
本文综述了蛋白质（免疫印迹法）印迹及其后续蛋白质分析所涉及的不同步骤。描述了蛋白质的电泳分离、转移至膜上的过程、免疫和非免疫蛋白质检测系统，以及蛋白质 - 核酸和蛋白质 - 蛋白质相互作用的表征。重点在于所描述方法的灵敏度以及可能的变化，这些变化可使免疫印迹法的各个步骤适用于特定问题。
